酿造珊瑚萜烯--基于酵母的软珊瑚萜烯环化酶研究方法。

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-04-04 DOI:10.1016/bs.mie.2024.03.023
Paul D Scesa, Eric W Schmidt
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引用次数: 0

摘要

珊瑚萜烯是用途广泛的重要分子。在这里,我们描述了一种大规模生产珊瑚萜烯支架的简便而可靠的方法。作为该方法的一个实例,我们在此发现、表达并鉴定了更多的 klysimplexin R 合成酶,从而扩展了已知的软珊瑚萜烯环化酶的酶学。我们希望所描述的基本方法能够促进对珊瑚萜烯及其生物合成基因功能的广泛基础研究,以及对生物医学和技术上重要分子的商业开发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Brewing coral terpenes-A yeast based approach to soft coral terpene cyclases.

Coral terpenes are important molecules with numerous applications. Here, we describe a robust and simple method to produce coral terpene scaffolds at scale. As an example of the approach, here we discover, express, and characterize further klysimplexin R synthases, expanding the known enzymology of soft coral terpene cyclases. We hope that the underlying method described will enable widespread basic research into the functions of coral terpenes and their biosynthetic genes, as well as the commercial development of biomedically and technologically important molecules.

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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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