牛数字皮炎中的噬菌体菌株感染期间,粘附和金属离子获取基因表达增加。

IF 2.9 3区 医学 Q3 IMMUNOLOGY
Infection and Immunity Pub Date : 2024-08-13 Epub Date: 2024-06-28 DOI:10.1128/iai.00117-24
Colton Scott, Angelica P Dias, Jeroen De Buck
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引用次数: 0

摘要

数字皮炎(DD)是奶牛足跟球部的一种溃疡性病变。DD 是一种多微生物疾病,没有确切的病因,但在病变组织中发现了大量的特雷波纳菌螺旋体。在特雷波纳菌中,有几个不同的种类可在 DD 中发现;然而,在疾病的活动期和溃疡期,特雷波纳菌中的噬菌体在皮肤深层大量存在。这些细菌在皮肤中存活的致病机制及其在 DD 病理学中的确切作用尚不清楚。为了探索噬菌体特雷波纳菌的致病机理和毒力,我们在小鼠皮下脓肿模型中对新分离出的噬菌体特雷波纳菌菌株进行了研究。在第一项试验中,进行了一项剂量研究,以脓肿体积为基础,比较不同菌株在三种不同的每接种体(TPI)特雷波数剂量下的致病性。在第二项试验中,获得了 11 个假定毒力基因的表达水平,以深入了解它们在致病过程中的参与情况。在 RT-qPCR 分析中,发现编码两种金属离子导入脂蛋白的基因和两种粘附基因在感染过程中高度上调。相反,在感染过程中,发现两个参与运动和趋化的基因没有明显上调或被利用。奶牛天然 M2 病变的基因表达数据支持了这些结果。这种基因表达分析可突出表明,T. phagedenis在策略上更倾向于在宿主体内存活和粘附,而不是参与运动和传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adherence and metal-ion acquisition gene expression increases during infection with Treponema phagedenis strains from bovine digital dermatitis.

Digital dermatitis (DD) is an ulcerative foot lesion on the heel bulbs of dairy cattle. DD is a polymicrobial disease with no precise etiology, although Treponema spirochetes are found disproportionally abundant in diseased tissue. Within Treponema, several different species are found in DD; however, the species Treponema phagedenis is uniformly found in copious quantities and deep within the skin layers of the active, ulcerative stages of disease. The pathogenic mechanisms these bacteria use to persist in the skin and the precise role they play in the pathology of DD are widely unknown. To explore the pathogenesis and virulence of Treponema phagedenis, newly isolated strains of this species were investigated in a subcutaneous murine abscess model. In the first trial, a dosage study was conducted to compare the pathogenicity of different strains across three different treponemes per inoculum (TPI) doses based on abscess volumes. In the second trial, the expression levels of 11 putative virulence genes were obtained to gain insight into their involvement in pathogenesis. During the RT-qPCR analysis, it was determined that genes encoding for two metal-ion import lipoproteins and two adherence genes were found highly upregulated during infection. Conversely, two genes involved in motility and chemotaxis were found to not be significantly upregulated or utilized during infection. These results were supported by gene expression data from natural M2 lesions of dairy cattle. This gene expression analysis could highlight the preference in strategy for T. phagedenis to persist and adhere in the host rather than engage in motility and disseminate.

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来源期刊
Infection and Immunity
Infection and Immunity 医学-传染病学
CiteScore
6.00
自引率
6.50%
发文量
268
审稿时长
3 months
期刊介绍: Infection and Immunity (IAI) provides new insights into the interactions between bacterial, fungal and parasitic pathogens and their hosts. Specific areas of interest include mechanisms of molecular pathogenesis, virulence factors, cellular microbiology, experimental models of infection, host resistance or susceptibility, and the generation of innate and adaptive immune responses. IAI also welcomes studies of the microbiome relating to host-pathogen interactions.
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