Jiahui Hu , Qianqian Wang , Yong Dai , Yueqin Tian , Nenggui Xu , Hongmei Wen , Zulin Dou , Qiuping Ye
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A poststroke dysphagia (PSD) model was established, and rats with dysphagia were grouped into three different intensities (0.1 mA, 0.5 mA, and 1 mA) for the selection of optimal intensity and three different frequencies (1 Hz, 2 Hz, and 5 Hz) for the selection of optimal frequency based on a stimulation duration of 10 min in the clinic. A Videofluroscopic Swallow Screen (VFSS) was used to assess swallowing function in rats before and after mPES treatment. The results showed that the 1 mA group had better swallowing function (<em>p < 0.05</em>) than the model group. Compared with the model group, the 1 Hz and 5 Hz groups had the same improvement in swallowing function (<em>p < 0.05</em>). However, the increase in excitatory signals in the sensorimotor cortex was more pronounced in the 5 Hz group than in the other frequency stimulation groups (<em>p < 0.05</em>). Combining the clinical findings with the above results, we concluded that the optimal stimulation parameter for mPES in rats is “frequency: 5 Hz, current intensity: 1 mA for 10 min/day”, which provides a basis for future basic experimental studies of mPES in animals.</p></div>","PeriodicalId":12246,"journal":{"name":"Experimental Neurology","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0014488624002048/pdfft?md5=4647f90a3161928e5cd535077ca292d4&pid=1-s2.0-S0014488624002048-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Screening for optimal parameters for modified pharyngeal electrical stimulation for the treatment of dysphagia after stroke in rats\",\"authors\":\"Jiahui Hu , Qianqian Wang , Yong Dai , Yueqin Tian , Nenggui Xu , Hongmei Wen , Zulin Dou , Qiuping Ye\",\"doi\":\"10.1016/j.expneurol.2024.114878\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Pharyngeal electrical stimulation (PES), a novel noninvasive peripheral nerve stimulation technique, can effectively improve neurogenic dysphagia and increase the safety and effectiveness of swallowing in the clinic. 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Compared with the model group, the 1 Hz and 5 Hz groups had the same improvement in swallowing function (<em>p < 0.05</em>). However, the increase in excitatory signals in the sensorimotor cortex was more pronounced in the 5 Hz group than in the other frequency stimulation groups (<em>p < 0.05</em>). 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引用次数: 0
摘要
咽部电刺激(PES)是一种新型的无创外周神经刺激技术,可有效改善神经源性吞咽困难,提高临床吞咽的安全性和有效性。然而,吞咽困难动物模型的缺乏限制了 PES 的机理研究,影响了其广泛应用。因此,需要确定大鼠 PES 的最佳参数,以便进行机理研究。改良型 PES(mPES)的波形和脉宽与 PES 不同;以往的研究发现,mPES 具有与 PES 相似的神经机制。建立了脑卒中后吞咽困难(PSD)模型,并将吞咽困难的大鼠分为三种不同的强度(0.1 mA、0.5 mA 和 1 mA),以选择最佳强度;三种不同的频率(1 Hz、2 Hz 和 5 Hz),以选择最佳频率。在 mPES 治疗前后,使用视频流变学吞咽筛查(VFSS)评估大鼠的吞咽功能。结果显示,1 毫安组的吞咽功能更好(p
Screening for optimal parameters for modified pharyngeal electrical stimulation for the treatment of dysphagia after stroke in rats
Pharyngeal electrical stimulation (PES), a novel noninvasive peripheral nerve stimulation technique, can effectively improve neurogenic dysphagia and increase the safety and effectiveness of swallowing in the clinic. However, the lack of animal models for dysphagia has limited the mechanistic research on PES, which affects its wide application. Therefore, determining optimal parameters for PES in rats is needed to enable mechanistic studies. Modified PES (mPES), which has different waves and pulse widths from PES, was used; in previous studies mPES was found to have a neurological mechanism like that of PES. A poststroke dysphagia (PSD) model was established, and rats with dysphagia were grouped into three different intensities (0.1 mA, 0.5 mA, and 1 mA) for the selection of optimal intensity and three different frequencies (1 Hz, 2 Hz, and 5 Hz) for the selection of optimal frequency based on a stimulation duration of 10 min in the clinic. A Videofluroscopic Swallow Screen (VFSS) was used to assess swallowing function in rats before and after mPES treatment. The results showed that the 1 mA group had better swallowing function (p < 0.05) than the model group. Compared with the model group, the 1 Hz and 5 Hz groups had the same improvement in swallowing function (p < 0.05). However, the increase in excitatory signals in the sensorimotor cortex was more pronounced in the 5 Hz group than in the other frequency stimulation groups (p < 0.05). Combining the clinical findings with the above results, we concluded that the optimal stimulation parameter for mPES in rats is “frequency: 5 Hz, current intensity: 1 mA for 10 min/day”, which provides a basis for future basic experimental studies of mPES in animals.
期刊介绍:
Experimental Neurology, a Journal of Neuroscience Research, publishes original research in neuroscience with a particular emphasis on novel findings in neural development, regeneration, plasticity and transplantation. The journal has focused on research concerning basic mechanisms underlying neurological disorders.