[不同运动模式对衰老大鼠神经肌肉接头和骨骼肌相关蛋白质代谢的影响]。

Q3 Medicine
生理学报 Pub Date : 2024-06-25
Yan-Hong Su, Yi Cheng, Ting-Ting Li, Yi-Chen Zhang, Ze-Yu DU, Juan Chen, Fu-Qing Wang, Zhong-Hao Liu, Wen-Han Gong
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引用次数: 0

摘要

本研究旨在探讨不同运动模式对衰老大鼠神经肌肉接头(NMJ)和骨骼肌相关蛋白代谢的影响。研究从38只雄性Sprague-Dawley(SD)大鼠(3月龄)中随机抽取10只分为年轻组(Y),其余大鼠饲养至21月龄后随机分为老年对照组(O)、耐力运动组(EN)和阻力运动组(R)。经过 8 周相应的运动训练后,采集大鼠的腓肠肌,用免疫荧光染色法检测许旺细胞中 S100B 的表达。采用免疫印迹法检测大鼠腓肠肌中凝集素蛋白(Agrin)、低密度脂蛋白受体相关蛋白4(Lrp4)、肌肉特异性激酶蛋白(MuSK)、下游酪氨酸激酶7(Dok7)、磷酸化蛋白激酶B(p-Akt)、磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)和磷酸化叉头盒O1(p-FoxO1)的蛋白表达水平。结果表明,耐力和阻力运动增加了老龄大鼠腓肠肌的湿重比。R组的S100B蛋白表达量明显高于O组和EN组。与 NMJ 功能相关的蛋白质,包括 Agrin、Lrp4、MuSK 和 Dok7,在 O 组明显低于 Y 组。阻力运动会上调衰老大鼠的这四种蛋白质,而耐力运动则无法逆转 Lrp4、MuSK 和 Dok7 的蛋白质表达水平。在骨骼肌相关蛋白方面,与Y组相比,O组的p-Akt和p-mTOR蛋白表达水平下调,p-FoxO1蛋白表达水平上调。阻力运动和耐力运动逆转了衰老大鼠 p-mTOR 和 p-FoxO1 蛋白表达的变化。这些研究结果表明,两种运动模式都能增强衰老大鼠的 NMJ 功能、增加蛋白质合成和减少骨骼肌相关蛋白质的分解代谢,其中阻力运动的效果更为明显。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Effects of different exercise modes on neuromuscular junction and metabolism of skeletal muscle-related proteins in aging rats].

The present study aimed to explore the effects of different exercise modes on neuromuscular junction (NMJ) and metabolism of skeletal muscle-related proteins in aging rats. Ten from 38 male Sprague-Dawley (SD) rats (3-month-old) were randomly selected into young (Y) group, while the rest were raised to 21 months old and randomly divided into elderly control (O), endurance exercise (EN) and resistance exercise (R) groups. After 8 weeks of corresponding exercises training, the gastrocnemius muscles of rats were collected, and the expression of S100B in Schwann cells was detected by immunofluorescence staining. Western blot was used to detect the protein expression levels of agglutinate protein (Agrin), low-density lipoprotein receptor-related protein 4 (Lrp4), muscle- specific kinase protein (MuSK), downstream tyrosine kinase 7 (Dok7), phosphorylated protein kinase B (p-Akt), phosphorylated mammalian target rapamycin (p-mTOR), and phosphorylated forkhead box O1 (p-FoxO1) in rat gastrocnemius muscles. The results showed that, endurance and resistance exercises increased the wet weight ratio of gastrocnemius muscle in the aging rats. The protein expression of S100B in the R group was significantly higher than those in the O and EN groups. Proteins related to NMJ function, including Agrin, Lrp4, MuSK, and Dok7 were significantly decreased in the O group compared with those in the Y group. Resistance exercise up-regulated these four proteins in the aging rats, whereas endurance exercise could not reverse the protein expression levels of Lrp4, MuSK and Dok7. Regarding skeletal muscle-related proteins, the O group showed down-regulated p-Akt, and p-mTOR protein expression levels and up-regulated p-FoxO1 protein expression level, compared to the Y group. Resistance and endurance exercises reversed the changes in p-mTOR and p-FoxO1 protein expression in the aging rats. These findings demonstrate that both exercise modes can enhance NMJ function, increase protein synthesis and reduce the catabolism of skeletal muscle-related proteins in aging rats, with resistance exercise showing a more pronounced effect.

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来源期刊
生理学报
生理学报 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
4820
期刊介绍: Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.
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