Kotaro Nakanishi, Keiichi Kojima, Yoshiyuki Sowa and Yuki Sudo*,
{"title":"利用微生物罗多普勒蛋白双向光学控制大肠杆菌中的质子动力","authors":"Kotaro Nakanishi, Keiichi Kojima, Yoshiyuki Sowa and Yuki Sudo*, ","doi":"10.1021/acs.jpcb.4c03027","DOIUrl":null,"url":null,"abstract":"<p >Proton (H<sup>+</sup>) motive force (PMF) serves as the energy source for the flagellar motor rotation, crucial for microbial motility. Here, to control PMF using light, we introduced light-driven inward and outward proton pump rhodopsins, <i>Rm</i>XeR and AR3, into <i>Escherichia coli</i>. The motility of <i>E. coli</i> cells expressing <i>Rm</i>XeR and AR3 significantly decreased and increased upon illumination, respectively. Tethered cell experiments revealed that, upon illumination, the torque of the flagellar motor decreased to nearly zero (28 pN nm) with <i>Rm</i>XeR, while it increased to 1170 pN nm with AR3. These alterations in PMF correspond to +146 mV (<i>Rm</i>XeR) and −140 mV (AR3), respectively. Thus, bidirectional optical control of PMF in <i>E. coli</i> was successfully achieved by using proton pump rhodopsins. This system holds a potential for enhancing our understanding of the roles of PMF in various biological functions.</p>","PeriodicalId":60,"journal":{"name":"The Journal of Physical Chemistry B","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bidirectional Optical Control of Proton Motive Force in Escherichia coli Using Microbial Rhodopsins\",\"authors\":\"Kotaro Nakanishi, Keiichi Kojima, Yoshiyuki Sowa and Yuki Sudo*, \",\"doi\":\"10.1021/acs.jpcb.4c03027\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Proton (H<sup>+</sup>) motive force (PMF) serves as the energy source for the flagellar motor rotation, crucial for microbial motility. Here, to control PMF using light, we introduced light-driven inward and outward proton pump rhodopsins, <i>Rm</i>XeR and AR3, into <i>Escherichia coli</i>. The motility of <i>E. coli</i> cells expressing <i>Rm</i>XeR and AR3 significantly decreased and increased upon illumination, respectively. Tethered cell experiments revealed that, upon illumination, the torque of the flagellar motor decreased to nearly zero (28 pN nm) with <i>Rm</i>XeR, while it increased to 1170 pN nm with AR3. These alterations in PMF correspond to +146 mV (<i>Rm</i>XeR) and −140 mV (AR3), respectively. Thus, bidirectional optical control of PMF in <i>E. coli</i> was successfully achieved by using proton pump rhodopsins. This system holds a potential for enhancing our understanding of the roles of PMF in various biological functions.</p>\",\"PeriodicalId\":60,\"journal\":{\"name\":\"The Journal of Physical Chemistry B\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Physical Chemistry B\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jpcb.4c03027\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Physical Chemistry B","FirstCategoryId":"1","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jpcb.4c03027","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Bidirectional Optical Control of Proton Motive Force in Escherichia coli Using Microbial Rhodopsins
Proton (H+) motive force (PMF) serves as the energy source for the flagellar motor rotation, crucial for microbial motility. Here, to control PMF using light, we introduced light-driven inward and outward proton pump rhodopsins, RmXeR and AR3, into Escherichia coli. The motility of E. coli cells expressing RmXeR and AR3 significantly decreased and increased upon illumination, respectively. Tethered cell experiments revealed that, upon illumination, the torque of the flagellar motor decreased to nearly zero (28 pN nm) with RmXeR, while it increased to 1170 pN nm with AR3. These alterations in PMF correspond to +146 mV (RmXeR) and −140 mV (AR3), respectively. Thus, bidirectional optical control of PMF in E. coli was successfully achieved by using proton pump rhodopsins. This system holds a potential for enhancing our understanding of the roles of PMF in various biological functions.
期刊介绍:
An essential criterion for acceptance of research articles in the journal is that they provide new physical insight. Please refer to the New Physical Insights virtual issue on what constitutes new physical insight. Manuscripts that are essentially reporting data or applications of data are, in general, not suitable for publication in JPC B.