Mei5-Sae3复合体亚基Mei5在酵母减数分裂过程中Dmc1介导的重组中的突变分析

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Stephen Mwaniki, Priyanka Sawant, Osaretin P. Osemwenkhae, Yurika Fujita, Masaru Ito, Asako Furukohri, Akira Shinohara
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引用次数: 0

摘要

减数分裂中的同源重组是由Dmc1重组酶介导的。酿酒酵母的 Mei5-Sae3 复合物可促进 Dmc1 的组装,并与 Dmc1 一起发挥同源介导的减数分裂 DNA 双链断裂修复功能。人们对Mei5-Sae3如何促进Dmc1组装仍知之甚少。在这项研究中,我们创建并鉴定了几个以基本残基的氨基酸置换为特征的mei5突变体。我们发现,Mei5 的 Arg97 在其同源物 SFR1(与 SWI5 的复合物)、RAD51 介体、人类和其他生物中是保守的,它是与 Sae3 形成复合物以组装 Dmc1 的关键。此外,在酵母减数分裂过程中,用Ala取代Mei5中的Arg117或Lys133会产生C端截短的Mei5蛋白。值得注意的是,当表达较短的Mei5-R117A蛋白时,在减数分裂细胞中观察到,而在有丝分裂细胞中观察不到,这表明Mei5-Sae3的翻译后加工对Dmc1介导的重组有独特的调节作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis

Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis

Interhomolog recombination in meiosis is mediated by the Dmc1 recombinase. The Mei5-Sae3 complex of Saccharomyces cerevisiae promotes Dmc1 assembly and functions with Dmc1 for homology-mediated repair of meiotic DNA double-strand breaks. How Mei5-Sae3 facilitates Dmc1 assembly remains poorly understood. In this study, we created and characterized several mei5 mutants featuring the amino acid substitutions of basic residues. We found that Arg97 of Mei5, conserved in its ortholog, SFR1 (complex with SWI5), RAD51 mediator, in humans and other organisms, is critical for complex formation with Sae3 for Dmc1 assembly. Moreover, the substitution of either Arg117 or Lys133 with Ala in Mei5 resulted in the production of a C-terminal truncated Mei5 protein during yeast meiosis. Notably, the shorter Mei5-R117A protein was observed in meiotic cells but not in mitotic cells when expressed, suggesting a unique regulation of Dmc1-mediated recombination by posttranslational processing of Mei5-Sae3.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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