PET/CMR 联合疗法显示的心肌梗死区域[18F]FDG 摄取增加与 snRNA-seq 检测到的炎症细胞入侵相关。

IF 7.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS
Basic Research in Cardiology Pub Date : 2024-10-01 Epub Date: 2024-06-26 DOI:10.1007/s00395-024-01064-y
Dominika Lukovic, Mariann Gyöngyösi, Imre J Pavo, Julia Mester-Tonczar, Patrick Einzinger, Katrin Zlabinger, Nina Kastner, Andreas Spannbauer, Denise Traxler, Noemi Pavo, Georg Goliasch, Dietmar Pils, Andras Jakab, Zsuzsanna Szankai, Ina Michel-Behnke, Lu Zhang, Yvan Devaux, Senta Graf, Dietrich Beitzke, Johannes Winkler
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引用次数: 0

摘要

[18F]FDG正电子发射计算机断层显像-心脏磁共振成像(PET/CMR)是评估急性心肌梗死(AMI)患者心肌活力和心脏功能的有效工具。在此,我们评估了 PET/CMR 在猪闭胸再灌注急性心肌梗死(rAMI)模型中的预后价值。在大多数动物(Match 组,n = 28)中,PET/CMR 成像的晚期钆增强显示梗死部位在 rAMI 后 3 天出现示踪剂摄取缺陷。在梗死面积较大、左室射血分数(LVEF)较差的动物中,观察到 rAMI 3 天后梗死部位的[18F]FDG 摄取增加(代谢/收缩力不匹配),而远端存活心肌的示踪剂摄取减少(不匹配组,n = 12)(34 ± 8.7 vs 42.0 ± 5.2%),随访 1 个月时 LVEF 也较低(35.8 ± 9.5 vs 43.0 ± 6.3%)。通过对梗死心肌和边缘区(每组 3 例,假手术对照组 3 例)进行大量和单核 RNA 测序进行转录组分析,发现 Mismatch 动物的梗死区和边缘区存在强烈的炎症反应,并有单核细胞和巨噬细胞浸润。我们的数据表明,在 rAMI 的亚急性阶段联合 PET/MRI 对随后的心脏功能损害具有高度的预后相关性,并强调了在 rAMI 后的初始阶段先天性免疫系统过度激活的不利影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Increased [<sup>18</sup>F]FDG uptake in the infarcted myocardial area displayed by combined PET/CMR correlates with snRNA-seq-detected inflammatory cell invasion.

Increased [18F]FDG uptake in the infarcted myocardial area displayed by combined PET/CMR correlates with snRNA-seq-detected inflammatory cell invasion.

Combined [18F]FDG PET-cardiac MRI imaging (PET/CMR) is a useful tool to assess myocardial viability and cardiac function in patients with acute myocardial infarction (AMI). Here, we evaluated the prognostic value of PET/CMR in a porcine closed-chest reperfused AMI (rAMI) model. Late gadolinium enhancement by PET/CMR imaging displayed tracer uptake defect at the infarction site by 3 days after the rAMI in the majority of the animals (group Match, n = 28). Increased [18F]FDG uptake at the infarcted area (metabolism/contractility mismatch) with reduced tracer uptake in the remote viable myocardium (group Mismatch, n = 12) 3 days after rAMI was observed in the animals with larger infarct size and worse left ventricular ejection fraction (LVEF) (34 ± 8.7 vs 42.0 ± 5.2%), with lower LVEF also at the 1-month follow-up (35.8 ± 9.5 vs 43.0 ± 6.3%). Transcriptome analyses by bulk and single-nuclei RNA sequencing of the infarcted myocardium and border zones (n = 3 of each group, and 3 sham-operated controls) revealed a strong inflammatory response with infiltration of monocytes and macrophages in the infarcted and border areas in Mismatch animals. Our data indicate a high prognostic relevance of combined PET/MRI in the subacute phase of rAMI for subsequent impairment of heart function and underline the adverse effects of an excessive activation of the innate immune system in the initial phase after rAMI.

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来源期刊
Basic Research in Cardiology
Basic Research in Cardiology 医学-心血管系统
CiteScore
16.30
自引率
5.30%
发文量
54
审稿时长
6-12 weeks
期刊介绍: Basic Research in Cardiology is an international journal for cardiovascular research. It provides a forum for original and review articles related to experimental cardiology that meet its stringent scientific standards. Basic Research in Cardiology regularly receives articles from the fields of - Molecular and Cellular Biology - Biochemistry - Biophysics - Pharmacology - Physiology and Pathology - Clinical Cardiology
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