Andrew Franklin, Alexa Freedman, Ann Borders, Lauren Keenan Devlin, Erin S Proctor, Erica Price, Steve Cole, Greg Miller, Linda M Ernst
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引用次数: 0
摘要
背景:胎盘母体血管灌注不良(MVM)的特点是绒毛成熟加速,并与抗衰老蛋白α-klotho(AK)的减少有关。我们的目的是确定胎盘和胎儿器官中 AK 蛋白和基因表达的特征:方法:我们利用了两个队列。首先,我们对尸检队列中的 AK 蛋白表达进行了特征描述,与死胎对照组相比,尸检队列中的病例被定义为 MVM 作为胎儿死亡的原因。其次,我们对患有和未患有 MVM 的活产婴儿的胎盘和脐带血 AK 基因表达进行了鉴定:结果:我们发现暴露于严重MVM的胎盘绒毛滋养细胞中蛋白质表达减少,暴露于MVM的胎盘组织中AK基因表达减少。我们没有发现胎儿器官或脐带血中的 AK 表达因存在或不存在 MVM 而存在统计学意义上的显著差异。此外,在活产婴儿中,我们还发现胎盘 AK 表达较低会增加早产的几率:结论:在 MVM 的情况下,胎盘中 AK 基因和蛋白表达的降低与 MVM 中胎盘老化的理论一致,并且与早产几率增加有关。
Decreased Alpha Klotho Expression in Placentas Exposed to Severe Maternal Vascular Malperfusion.
Background: Placental maternal vascular malperfusion (MVM) is characterized by accelerated villous maturation and has been associated with a decrease in the antiaging protein, alpha-klotho (AK). Our aim was to characterize AK protein and gene expression in the placenta and fetal organs.
Methods: We utilized 2 cohorts. First, we characterized AK protein expression in an autopsy cohort where cases were defined as MVM as the cause of fetal death compared to a stillborn control population. Second, we characterized placental and umbilical cord blood AK gene expression in a liveborn population with and without MVM.
Results: We found decreased protein expression in the villous trophoblastic cells of placentas exposed to severe MVM and decreased AK gene expression in placental tissue exposed to MVM. We did not see any statistically significant differences in fetal organ or umbilical cord blood AK expression based on the presence or absence of MVM. Furthermore, in liveborn infants, we also found increased odds of preterm birth with lower placental AK expression.
Conclusions: Decreased AK gene and protein expression in the placenta in the setting of MVM is consistent with the theory of placental aging in MVM and is associated with increased odds of preterm birth.
期刊介绍:
The Journal covers the spectrum of disorders of early development (including embryology, placentology, and teratology), gestational and perinatal diseases, and all diseases of childhood. Studies may be in any field of experimental, anatomic, or clinical pathology, including molecular pathology. Case reports are published only if they provide new insights into disease mechanisms or new information.