全球携带 blaNDM 大肠杆菌的流行病学和基因组特征。

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Changyu Xia, Ruyu Yan, Chang Liu, Junbin Zhai, Jie Zheng, Wei Chen, Xiaoli Cao
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引用次数: 0

摘要

背景:大肠埃希菌是新德里金属-β-内酰胺酶(NDM)最常见的宿主,该酶可水解除阿曲南外的几乎所有β-内酰胺类药物。携带 blaNDM 的大肠杆菌在全球范围内的传播严重威胁着公众健康:本研究旨在探索携带 blaNDM 的大肠杆菌分离株的全球基因组流行病学,为防止此类菌株的传播提供信息:方法:从 NCBI 数据库下载全球大肠杆菌基因组,使用 BLASTP 检测 blaNDM。使用 Per 软件从 GenBank 中提取有关宿主、资源、采集数据和原产国的元信息。利用 CLC Workbench 分析了序列类型(STs)和抗菌药耐药性基因(ARG)的分布;通过向网站提交基因组分析了质粒复制子、血清型和毒力基因(VFs)。结果显示,截至2023年3月,共有1,770个ARGs和1,770个质粒复制子被发现:截至2023年3月,在2003-2022年期间收集的33 055株分离株中,共发现1 774株含有blaNDM。其中,发现了 15 个 blaNDM 变体,最常见的是 blaNDM-5(74.1%),其次是 blaNDM-1(16.6%)和 blaNDM-9(4.6%)。在发现的 213 个 ARGs 中,发现了 27 个 blaCTX-M 和 39 个 blaTEM 变体,其中 blaCTX-M-15 (438 个,24.7%)和 blaTEM-1B (1092 个,61.6%)分别是最常见的变体。此外,还检测到 546 个(30.8%)质粒介导的 ampC 基因、508 个(28.6%)外源获得的 16 S rRNA 甲基转移酶编码基因和 262 个(14.8%)mcr。在 232 种不同的 ST 中,ST167(17.2%)最为普遍。质粒方面,半数以上的分离株含有 IncFII、IncFIB 和 IncX3。VF terC、gad、traT 和 iss 以及血清型 O101:H9(n = 231,13.0%)、O8:H9(n = 115,6.5%)和 O9:H30(n = 99,5.6%)也经常被观察到:该研究深入探讨了质粒类型、毒力因子和 ARGs 之间错综复杂的关系,为临床治疗和公共卫生干预提供了宝贵的见解,是指导未来研究、监测和实施有效策略以应对携带 blaNDM 的大肠杆菌所带来的挑战的重要资源。研究结果突出表明,迫切需要开展持续的全球合作、监测工作和抗菌药物管理,以减轻这些高耐药性菌株对公共卫生的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epidemiological and genomic characteristics of global blaNDM-carrying Escherichia coli.

Background: Escherichia. coli is the most frequent host for New Delhi metallo-β-lactamase (NDM) which hydrolyzes almost all β-lactams except aztreonam. The worldwide spread of blaNDM-carrying E. coli heavily threatens public health.

Objective: This study aimed to explore the global genomic epidemiology of blaNDM- carrying E. coli isolates, providing information for preventing the dissemination of such strains.

Methods: Global E. coli genomes were downloaded from NCBI database and blaNDM was detected using BLASTP. Per software was used to extract meta information on hosts, resources, collection data, and countries of origin from GenBank. The sequence types (STs) and distribution of antimicrobial resistance gene (ARG) were analyzed by CLC Workbench; Plasmid replicons, serotypes and virulence genes (VFs) were analyzed by submitting the genomes to the websites. Statistical analyses were performed to access the relationships among ARGs and plasmid replicons.

Results: Until March 2023, 1,774 out of 33,055 isolates collected during 2003-2022 were found to contain blaNDM in total. Among them, 15 blaNDM variants were found with blaNDM-5 (74.1%) being most frequent, followed by blaNDM-1 (16.6%) and blaNDM-9 (4.6%). Among the 213 ARGs identified, 27 blaCTX-M and 39 blaTEM variants were found with blaCTX-M-15 (n = 438, 24.7%) and blaTEM-1B (n = 1092, 61.6%) being the most frequent ones, respectively. In addition, 546 (30.8%) plasmids mediated ampC genes, 508 (28.6%) exogenously acquired 16 S rRNA methyltransferase encoding genes and 262 (14.8%) mcr were also detected. Among the 232 distinct STs, ST167 (17.2%) were the most prevalent. As for plasmids, more than half of isolates contained IncFII, IncFIB and IncX3. The VF terC, gad, traT and iss as well as the serotypes O101:H9 (n = 231, 13.0%), O8:H9 (n = 115, 6.5%) and O9:H30 (n = 99, 5.6%) were frequently observed.

Conclusions: The study delves into the intricate relationship between plasmid types, virulence factors, and ARGs, which provides valuable insights for clinical treatment and public health interventions, and serves as a critical resource for guiding future research, surveillance, and implementation of effective strategies to address the challenges posed by blaNDM-carrying E. coli. The findings underscore the urgent need for sustained global collaboration, surveillance efforts, and antimicrobial stewardship to mitigate the impact of these highly resistant strains on public health.

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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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