Control of cellular and viral transcription during adenovirus infection.

The control of transcription initiation is an issue central to the regulation of eukaryotic gene expression, and as such, the elucidation of the mechanisms of control of initiation frequency is critical. The study of adenovirus transcription control has provided insights into these mechanisms. Transcription of the early viral genes is activated by the product of the viral E1A gene. Possibly of greater importance is the fact that this activation does not appear to be "viral specific". Rather, the E1A protein effects a general activation of transcription in the cell, resulting in the stimulation of transcription of at least one cellular gene in addition to the viral genes. Furthermore, there appears to be a cellular activity that functions in a manner analogous to E1A. Recent experiments also suggest a role for E1A in negative regulation of transcription, mediated through enhancer elements, that may be one aspect of gene control during cellular differentiation. Therefore, the study of E1A action may well contribute to an understanding of cellular transcription control. Finally, other mechanisms of transcription control in adenovirus infected cells such as genome replication-dependent gene activation and transcription termination control will likely contribute to the overall understanding of the control of mammalian cell gene expression.