结合蛋白质组学和转录组学的分析揭示了海参自溶的调控目标

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Tingting Yan , Jinghe Sun , Jie Zheng , Jingfeng Yang
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引用次数: 0

摘要

海参是一种珍贵的海产品,自溶是水产养殖业的主要问题。本研究采用蛋白质组学和转录组学研究海参的自溶机制。将新鲜海参置于紫外线下诱导自溶。切取体壁样本进行蛋白质组学和转录组学分析。分别对活海参灌胃血管紧张素转换酶(ACE)抑制剂特普罗肽和伊马替尼激活剂,以确定调控靶标。通过外观、可溶性肽和羟脯氨酸含量来评估自溶的发生。四个基因-蛋白对分别是 ACE、AJAP10923、血红素结合蛋白 2-like、Ficolin-2-like。只有 ACE 蛋白和基因同步发生了变化,自溶海参中的 ACE 显著下调。替普罗泰导致 TCA 可溶性蛋白含量增加了 1.58 倍,羟脯氨酸含量增加了 1.57 倍。伊马替尼处理的海参与新鲜海参在 TCA 可溶性蛋白含量和羟脯氨酸含量方面没有明显差异(P > 0.05)。ACE 抑制剂加速了海参的自溶,而 ACE 激活剂则抑制了自溶。因此,ACE可作为海参自溶的调节靶标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An analysis combining proteomics and transcriptomics revealed a regulation target of sea cucumber autolysis

An analysis combining proteomics and transcriptomics revealed a regulation target of sea cucumber autolysis

Sea cucumber is a valuable seafood product and autolysis is the main concern for the aquaculture industry. This study employed proteomics and transcriptomics to investigate the autolysis mechanism of sea cucumbers. The fresh sea cucumber was exposed to UV light to induce autolysis. The body wall samples were cut off to analyze by proteomics and transcriptomics. The angiotensin-converting enzyme (ACE) inhibitor of teprotide and the activator of imatinib were gastric gavage to live sea cucumbers, respectively, to identify the regulation target. Autolysis occurrence was evaluated by appearance, soluble peptide, and hydroxyproline content. Four gene-protein pairs were ACE, AJAP10923, Heme-binding protein 2-like, and Ficolin-2-like. Only the ACE protein and gene changed synchronously and a significant down-regulation of ACE occurred in the autolysis sea cucumbers. Teprotide led to a 1.58-fold increase in the TCA-soluble protein content and a 1.57-fold increase in hydroxyproline content. No significant differences were observed between imatinib-treated sea cucumbers and fresh ones regarding TCA-soluble protein content or hydroxyproline levels (P > 0.05). ACE inhibitor accelerated the autolysis of sea cucumber, but ACE activator inhibited the autolysis. Therefore, ACE can serve as a regulatory target for autolysis in sea cucumbers.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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