元基因组分析揭示了污水处理对印度班加罗尔微生物组和抗生素耐药性组的不同影响

IF 4.3 4区 环境科学与生态学 Q2 ENGINEERING, ENVIRONMENTAL
Water Reuse Pub Date : 2024-06-06 DOI:10.2166/wrd.2024.032
Annamalai Nataraj, D. Mondhe, Vishwananth Srikantaiah, F. Ishtiaq
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引用次数: 0

摘要

气候变化和健康与用于灌溉的城市污水密切相关。污水处理厂(STP)为抗生素耐药基因(ARGs)在病原菌和非病原菌之间的传播提供了理想的环境和生态位。在这项研究中,我们考察了印度班加罗尔城市污水管网中 26 个污水处理厂从进水口到出水口的污水处理方法对微生物多样性和抗生素耐药基因组的不同影响。我们筛选了 478 个 ARGs,在废水中发现了 273 个 ARGs,其中包括与临床相关的基因,如 CTX-M、qnr、sul-1 和 NDM-1,这些基因可产生对六大类抗生素的耐药性。与排放口相比,污水入口处的 ARGs 含量更高。我们观察到,除了氨基糖苷类、β-内酰胺类、MLSB 和四环素外,从进水口样本到出水口样本,药物类别呈下降趋势。与出口样本相比,入口样本的 ARGs 与细菌之间的相关性更为复杂。我们的研究结果可作为一项基线研究,有助于对可培养和不可培养类群的基因进行量化,并有助于制定政策和策略,解决与循环水使用相关的水质问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metagenomic analysis reveals differential effects of sewage treatment on the microbiome and antibiotic resistome in Bengaluru, India
Climate change and health are closely linked to urban wastewater used for irrigation. Sewage treatment plants (STPs) provide ideal environments and niche availability for the transmission of antibiotic resistance genes (ARGs) among pathogenic and non-pathogenic bacteria. In this study, we examined the differential effect of sewage processing methods from the inlet to the outlet on the microbial diversity and antibiotic resistomes of 26 STPs in the urban sewage network of Bengaluru, India. We screened 478 ARGs and found 273 ARGs in wastewater, including clinically relevant genes such as CTX-M, qnr, sul-1, and NDM-1, which confer resistance to six major classes of antibiotics. The richness of ARGs was higher in sewage inlets compared with outlets. We observed a downward shift in drug classes from inlet to outlet samples, except for aminoglycosides, beta-lactams, MLSB, and tetracycline. Inlet samples exhibited more complex correlations between ARGs and bacteria compared with outlet samples. Our findings serve as a baseline study that could aid in the quantification of genes from both culturable and non-culturable taxa and will assist in the development of policies and strategies to address water quality issues associated with the use of recycled water.
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来源期刊
Water Reuse
Water Reuse Multiple-
CiteScore
6.20
自引率
8.90%
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0
审稿时长
7 weeks
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