K. Prasanth, D. C. Lakshmana Reddy, S. Sriram, R. Venugopalan, M. Pitchaimuthu, K. Hima Bindu, B. Varalakshmi
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引用次数: 0
摘要
通过与两个易感品系 Arka Harit 和 IIHR Sel-5-8 杂交,研究了苦瓜抗性品系 IIHR-144-1 对白粉病的抗性遗传。F2 后代和回交后代的分离比表明,抗性受显性-隐性外显或显性-抑制性外显的支配,这表明抗性品系 IIHR-144-1 中涉及多对基因,一对显性基因和一对隐性基因以及其他背景次要基因。利用 SSR 标记对 IIHR-144-1 与 Arka Harit 杂交的 F2 群体进行了大量分离分析,发现 McSSR 57 产生了一个多态性 DNA 片段,与疾病反应存在共分离。考虑到 Momordica charantia L. 与 M. charantia var. muricata(IIHR-144-1)之间的杂交相容性,抗性性状可通过杂交和选择转移到栽培品种上。
Genetic analysis and identification of SSR marker linked topowdery mildew resistance in bitter gourd (Momordica charantia L.)
The inheritance of powdery mildew resistance in bitter gourd resistant line IIHR-144-1 was studied in cross with two susceptible lines Arka Harit and IIHR Sel-5-8. The segregation ratios in F2 and backcross progenies indicated that resistance is governed by dominant-recessive epistasis or dominant inhibitory epistasis, indicating the involvement of more than one pairs of genes, one dominant and one recessive for resistance along with other background minor genes in the resistant line IIHR-144-1. Bulk segregant analysis of F2 population of cross between IIHR-144-1 and Arka Harit using SSR markers identified McSSR 57 produced a polymorphic DNA fragment that co-segregated with disease reaction. Considering the cross compatibility between Momordica charantia L. and M. charantia var. muricata (IIHR-144-1) the resistance trait can be transferred to cultivated species through hybridization and selection.