不同泌乳期和饲养方式的奶牛蛋白质特征

S. Kolomiets, L. Sosnina
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摘要

目的:研究高食量奶牛的蛋白质代谢规律,这取决于在俄罗斯欧洲北部条件下以附着和非附着方式饲养动物的泌乳期。生化研究的目的是在动物生物化学和生理学实验室的基础上,利用 Volnz RAS FSBBUN 农业研究和生物技术中心农业研究和生物技术中心的设备确定动物的蛋白质概况。研究对象是家庭生产力超过 8500 千克的 Holstinized 品种黑栖息奶牛。在选择实验动物组别时,考虑了生理状态、性周期阶段和内容物的方法。共有 108 头奶牛参加了实验。早上在饲喂前从尾静脉采血。饲喂条件和内容符合动物的品种特征,确保了高生产率指标的发展。为了评估蛋白质代谢状况,测定了血清中的含量:总蛋白;白蛋白;α1球蛋白;α2球蛋白;β球蛋白;γ球蛋白;蛋白质指数(白蛋白与球蛋白的比率)。研究材料为血清。总共调查了 108 份样本。有经验的动物血清中总蛋白质的浓度在相当稳定的范围内,在所研究的动物群体中,其偏差比生理周期中的既定标准低 1%至 5%。研究动物血清中总蛋白质和蛋白质组分的含量与截留方法之间存在联系。因此,在泌乳期的所有阶段,采用绑定法检测的总蛋白、蛋白指数和白蛋白指标都高于采用非绑定法检测的指标。在酒店后(泌乳期 1-100 天),总蛋白和伽马球蛋白含量的差异最大,达到 5%。干奶期动物的蛋白质指标对含量方法的依赖性最小。总蛋白含量分别为 8.0 和 7.99 g%,白蛋白含量分别为 3.07 和 3.04 g%,蛋白质指数分别为 0.6 和 0.58,采用的是结合型和非结合型截留方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Features of the protein profile of cows depending on the lactation period and method of housing
Purpose: the study of the laws of protein metabolism of high -eating cows, depending on the period of lactation in the attached and unmistable ways of keeping animals in the conditions of the European North of Russia.Materials and methods. Biochemical studies were conducted with the aim of determining the protein profile of animals on the basis of the laboratory of biochemistry and physiology of animals using the equipment of the Center for Agricultural Research and Biotechnology Center for the Center for Agricultural Studies and Biotechnology, the Volnz RAS FSBBUN. The object of the study was the cows of the black-and-habitat of the Holstinized breed with a productivity of over 8500 kg in the household. When selecting groups of experimental animals, the physiological state, the stage of the sexual cycle and the method of content were taken into account. In total, 108 cows participated in the experience. Blood was taken from the tail vein in the morning in front of feeding. The conditions of feeding and content corresponded to the breed features of animals and ensured the development of high productivity indicators. To assess the condition of protein metabolism, the content was determined in the blood serum: total protein; Albuminov; alpha 1 globulin; Alpha 2 globulins; Beta Globulinov; Gamma Globulinov; protein index (the ratio of albumins to globulin). The material for the study was blood serum. In total, 108 samples were investigated.Results. The concentration of total protein in the blood serum of experienced animals is within fairly constant limits and the deviation is from 1 to 5 % lower than the established norm during the physiological cycle in the studied groups of animals. A connection has been established between the content of total protein and protein fractions in the serum of the studied animals and the method of detention. So the indicators of the total protein, the protein index and albumines with the tied method of content are higher than with the unmistable method of content during all periods of lactation. The maximum difference was detected in the period after the hotel (1-100 days of lactation) by the content of total protein and gamma globulin and amounted to 5 %. The smallest dependence of the indicators of the protein profile on the method of content was detected in animals during dryness. The level of total protein amounted to 8.0 and 7.99 g%, the content of albumins 3.07 and 3.04 g%, the protein index 0.6 and 0.58, with a binding and unmistable method of detention, respectively.
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