{"title":"不同泌乳期和饲养方式的奶牛蛋白质特征","authors":"S. Kolomiets, L. Sosnina","doi":"10.31043/2410-2733-2024-1-54-59","DOIUrl":null,"url":null,"abstract":"Purpose: the study of the laws of protein metabolism of high -eating cows, depending on the period of lactation in the attached and unmistable ways of keeping animals in the conditions of the European North of Russia.Materials and methods. Biochemical studies were conducted with the aim of determining the protein profile of animals on the basis of the laboratory of biochemistry and physiology of animals using the equipment of the Center for Agricultural Research and Biotechnology Center for the Center for Agricultural Studies and Biotechnology, the Volnz RAS FSBBUN. The object of the study was the cows of the black-and-habitat of the Holstinized breed with a productivity of over 8500 kg in the household. When selecting groups of experimental animals, the physiological state, the stage of the sexual cycle and the method of content were taken into account. In total, 108 cows participated in the experience. Blood was taken from the tail vein in the morning in front of feeding. The conditions of feeding and content corresponded to the breed features of animals and ensured the development of high productivity indicators. To assess the condition of protein metabolism, the content was determined in the blood serum: total protein; Albuminov; alpha 1 globulin; Alpha 2 globulins; Beta Globulinov; Gamma Globulinov; protein index (the ratio of albumins to globulin). The material for the study was blood serum. In total, 108 samples were investigated.Results. The concentration of total protein in the blood serum of experienced animals is within fairly constant limits and the deviation is from 1 to 5 % lower than the established norm during the physiological cycle in the studied groups of animals. A connection has been established between the content of total protein and protein fractions in the serum of the studied animals and the method of detention. So the indicators of the total protein, the protein index and albumines with the tied method of content are higher than with the unmistable method of content during all periods of lactation. The maximum difference was detected in the period after the hotel (1-100 days of lactation) by the content of total protein and gamma globulin and amounted to 5 %. The smallest dependence of the indicators of the protein profile on the method of content was detected in animals during dryness. The level of total protein amounted to 8.0 and 7.99 g%, the content of albumins 3.07 and 3.04 g%, the protein index 0.6 and 0.58, with a binding and unmistable method of detention, respectively.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"136 5","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Features of the protein profile of cows depending on the lactation period and method of housing\",\"authors\":\"S. Kolomiets, L. Sosnina\",\"doi\":\"10.31043/2410-2733-2024-1-54-59\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Purpose: the study of the laws of protein metabolism of high -eating cows, depending on the period of lactation in the attached and unmistable ways of keeping animals in the conditions of the European North of Russia.Materials and methods. Biochemical studies were conducted with the aim of determining the protein profile of animals on the basis of the laboratory of biochemistry and physiology of animals using the equipment of the Center for Agricultural Research and Biotechnology Center for the Center for Agricultural Studies and Biotechnology, the Volnz RAS FSBBUN. The object of the study was the cows of the black-and-habitat of the Holstinized breed with a productivity of over 8500 kg in the household. When selecting groups of experimental animals, the physiological state, the stage of the sexual cycle and the method of content were taken into account. In total, 108 cows participated in the experience. Blood was taken from the tail vein in the morning in front of feeding. The conditions of feeding and content corresponded to the breed features of animals and ensured the development of high productivity indicators. To assess the condition of protein metabolism, the content was determined in the blood serum: total protein; Albuminov; alpha 1 globulin; Alpha 2 globulins; Beta Globulinov; Gamma Globulinov; protein index (the ratio of albumins to globulin). The material for the study was blood serum. In total, 108 samples were investigated.Results. The concentration of total protein in the blood serum of experienced animals is within fairly constant limits and the deviation is from 1 to 5 % lower than the established norm during the physiological cycle in the studied groups of animals. A connection has been established between the content of total protein and protein fractions in the serum of the studied animals and the method of detention. So the indicators of the total protein, the protein index and albumines with the tied method of content are higher than with the unmistable method of content during all periods of lactation. The maximum difference was detected in the period after the hotel (1-100 days of lactation) by the content of total protein and gamma globulin and amounted to 5 %. The smallest dependence of the indicators of the protein profile on the method of content was detected in animals during dryness. The level of total protein amounted to 8.0 and 7.99 g%, the content of albumins 3.07 and 3.04 g%, the protein index 0.6 and 0.58, with a binding and unmistable method of detention, respectively.\",\"PeriodicalId\":346303,\"journal\":{\"name\":\"Genetics and breeding of animals\",\"volume\":\"136 5\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetics and breeding of animals\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31043/2410-2733-2024-1-54-59\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetics and breeding of animals","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31043/2410-2733-2024-1-54-59","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Features of the protein profile of cows depending on the lactation period and method of housing
Purpose: the study of the laws of protein metabolism of high -eating cows, depending on the period of lactation in the attached and unmistable ways of keeping animals in the conditions of the European North of Russia.Materials and methods. Biochemical studies were conducted with the aim of determining the protein profile of animals on the basis of the laboratory of biochemistry and physiology of animals using the equipment of the Center for Agricultural Research and Biotechnology Center for the Center for Agricultural Studies and Biotechnology, the Volnz RAS FSBBUN. The object of the study was the cows of the black-and-habitat of the Holstinized breed with a productivity of over 8500 kg in the household. When selecting groups of experimental animals, the physiological state, the stage of the sexual cycle and the method of content were taken into account. In total, 108 cows participated in the experience. Blood was taken from the tail vein in the morning in front of feeding. The conditions of feeding and content corresponded to the breed features of animals and ensured the development of high productivity indicators. To assess the condition of protein metabolism, the content was determined in the blood serum: total protein; Albuminov; alpha 1 globulin; Alpha 2 globulins; Beta Globulinov; Gamma Globulinov; protein index (the ratio of albumins to globulin). The material for the study was blood serum. In total, 108 samples were investigated.Results. The concentration of total protein in the blood serum of experienced animals is within fairly constant limits and the deviation is from 1 to 5 % lower than the established norm during the physiological cycle in the studied groups of animals. A connection has been established between the content of total protein and protein fractions in the serum of the studied animals and the method of detention. So the indicators of the total protein, the protein index and albumines with the tied method of content are higher than with the unmistable method of content during all periods of lactation. The maximum difference was detected in the period after the hotel (1-100 days of lactation) by the content of total protein and gamma globulin and amounted to 5 %. The smallest dependence of the indicators of the protein profile on the method of content was detected in animals during dryness. The level of total protein amounted to 8.0 and 7.99 g%, the content of albumins 3.07 and 3.04 g%, the protein index 0.6 and 0.58, with a binding and unmistable method of detention, respectively.