Laboratory Study to evaluate the efficiency of Nano-loaded tobacco aqueous extract on the different roles of Ommatissus lybicus De Berg

A laboratory study is conducted to evaluate the efficiency of the aqueous extract of nano- chitosan loaded tobacco plants and laboratory-made. When O.  lybicus eggs were treated with an aqueous extract of nano- chitosan loaded tobacco plants at two concentrations (10-15) ppm, recorded the highest mortality rates reached (21.2-37) %, respectively. Furthermore, the highest egg mortality rates reached (47.8_66.6) % after (14_21) days. The same concentrations above also affected the mortality rates of first-instar nymphs, which amounted to (30.3_53.3%), respectively. The concentration (15) ppm had the highest significant differences at mortality rate reaching (58.3_70.0) % after (5_7) days. The mortality rates in third-instar nymphs reached (28.9-37.5%) at the concentration of (15) ppm, the highest mortality rates reached (39.9_44.6_40.3) % after (3_5_7) days. The mortality rates in the fifth instar nymphs amounted to (22.6_29.1) %, respectively, as they were the highest mortality rates at a concentration of (15) ppm after (5) days, reaching (39.1) %.