高原大麦中的原花青素 B1 和香豆酸通过调节 PPARα 介导的糖尿病 C57BL/6J 小鼠肝脂代谢和肠道微生物群来缓解高脂饮食诱发的高脂血症

Foods Pub Date : 2024-06-12 DOI:10.3390/foods13121843
Zehua Liu, Jianshen Liu, Ruoxin Tang, Zhaowan Zhang, Shuangqi Tian
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引用次数: 0

摘要

全谷物高原大麦(WHB)膳食被认为具有缓解高脂血症的潜力,高脂血症的主要特征是血脂在血清和肝脏中的积累。此前,研究发现全谷物高原大麦中的原花青素 B1(PB)和香豆酸(CA)可减轻糖耐量受损小鼠的血清脂质积累,但其对肝脏脂质代谢的调节作用尚不清楚。本研究结果表明,在高脂饮食(HFD)诱导的C57BL/6J糖尿病小鼠肝细胞中,补充PB和CA能激活过氧化物酶体增殖激活受体α(PPARα)和胆固醇7-α羟化酶(CYP7A1)及肉碱棕榈酰转移酶I(Cpt1)靶基因的表达、从而降低血清总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白(LDL-C)的含量,并增加高密度脂蛋白(HDL-C)的含量。16S rRNA 的高通量测序表明,补充 PB 和 CA 可改善肠道微生物群失调,这与反刍球菌属相对丰度的降低以及乳酸杆菌、脱硫弧菌和 Akkermansia 相对丰度的升高有关。斯皮尔曼相关分析表明,这些菌属与肥胖相关指数密切相关。总之,来自 WHB 的 PB 和 CA 对 PPARα 表达的激活对缓解高脂血症和调整肠道微生物群结构非常重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Procyanidin B1 and Coumaric Acid from Highland Barley Alleviated High-Fat-Diet-Induced Hyperlipidemia by Regulating PPARα-Mediated Hepatic Lipid Metabolism and Gut Microbiota in Diabetic C57BL/6J Mice
A whole-grain highland barley (WHB) diet has been recognized to exhibit the potential for alleviating hyperlipidemia, which is mainly characterized by lipids accumulation in the serum and liver. Previously, procyanidin B1 (PB) and coumaric acid (CA) from WHB were found to alleviate serum lipid accumulation in impaired glucose tolerance mice, while the effect on modulating the hepatic lipid metabolism remains unknown. In this study, the results showed the supplementation of PB and CA activated the expression of peroxisome proliferator-activated receptor α (PPARα) and the target genes of cholesterol 7-α hydroxylase (CYP7A1) and carnitine palmitoyl transferase I (Cpt1) in the liver cells of high-fat-diet (HFD)-induced diabetic C57BL/6J mice, resulting in decreases in the serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL-C) contents, and an increase in the high-density lipoprotein (HDL-C) content. High-throughput sequencing of 16S rRNA indicated that supplementation with PB and CA ameliorated the gut microbiota dysbiosis, which was associated with a reduction in the relative abundance of Ruminococcaceae and an increase in the relative abundance of Lactobacillus, Desulfovibrio, and Akkermansia. Spearman’s correlation analysis revealed that these genera were closely related to obesity-related indices. In summary, the activation of PPARα expression by PB and CA from WHB was important for the alleviation of hyperlipidemia and the structural adjustment of the gut microbiota.
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