N-乙酰半胱氨酸脂质体降低铁超载诱导的人肾细胞毒性

IF 2.8 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology Pub Date : 2024-06-14 DOI:10.1002/jctb.7695

Doohee Lee, Chaewon Bae, Suhyun Ryu, Kangwon Lee

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引用次数: 0

摘要

体内铁超载会通过芬顿反应产生自由基，从而对各种器官造成不良影响。一些抗氧化剂被推荐用于有效治疗与铁有关的疾病。N-乙酰半胱氨酸（NAC）是一种强效抗氧化剂，本研究对其减轻铁超载诱导的细胞死亡的潜力进行了调查。研究人员开发了一种脂质体配方，利用反相蒸发法将 NAC 包裹起来，以提高其输送能力和效果。通过评估脂质体 NAC 的大小、zeta 电位、形态和释放曲线，对其进行了表征。在人肾 2（HK-2）细胞中评估了脂质体 NAC 对铁超载诱导毒性的保护作用。细胞活力检测（细胞计数试剂盒 8）和活性氧检测（DCFDA 检测）证实了脂质体 NAC 在抑制缓慢铁吸收（1 mmol L-1 枸橼酸铁铵）诱导的铁突变方面的有效性。此外，脂质体 NAC 还能保护细胞免受快速铁吸收（50 μmol L-1 枸橼酸铁铵、20 μmol L-1 8-羟基喹啉）引起的毒性。这些研究结果表明，与游离 NAC 相比，脂质体 NAC 能更有效地保护人类肾脏细胞免受不同铁摄取模型引起的毒性，从而凸显了脂质体 NAC 作为一种治疗铁超载引起的细胞损伤的优质疗法的潜力。作者：© 2024。化学技术与生物技术杂志》由约翰威利父子有限公司代表化学工业学会（SCI）出版。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

N-Acetyl cysteine-loaded liposomes to reduce iron overload-induced toxicity in human kidney cells

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N-Acetyl cysteine-loaded liposomes to reduce iron overload-induced toxicity in human kidney cells

BACKGROUND

Iron overload in the body generates free radicals through the Fenton reaction, leading to adverse effects on various organs. Several antioxidants have been recommended to treat iron-related diseases effectively. N-Acetyl cysteine (NAC), a potent antioxidant, was investigated in this study for its potential to mitigate iron overload-induced cell death. A liposomal formulation was developed to encapsulate NAC, employing the reverse phase evaporation method, to enhance its delivery and effectiveness. The liposomal NAC was characterized by evaluating its size, zeta potential, morphology and release profiles.

RESULTS

The protective effect of liposomal NAC against iron overload-induced toxicity was assessed in human kidney 2 (HK-2) cells. Cell viability assays (Cell Counting Kit 8) and reactive oxygen species assays (DCFDA assay) confirmed the effectiveness of liposomal NAC in inhibiting ferroptosis induced by slow iron uptake (1 mmol L⁻¹ ferric ammonium citrate). Additionally, liposomal NAC protected cells from toxicity resulting from rapid iron uptake (50 μmol L⁻¹ ferric ammonium citrate, 20 μmol L⁻¹ 8-hydroxyquinoline). The liposomal formulation demonstrated an enhanced protective effect compared to free NAC.

CONCLUSION

These findings suggest that liposomal NAC more effectively protects human kidney cells from toxicity caused by different iron uptake models than free NAC, highlighting its potential as a superior treatment option for iron overload-induced cellular damage. © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.