通过定量综合蛋白质组学和磷酸蛋白质组学分析揭示野生赤松对毛虫取食胁迫的防御反应

Tianhua Sun, Yanan Zhao, Guona Zhou, Suhong Gao, Junxia Liu, Baojia Gao
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引用次数: 0

摘要

松属具有重要的经济和生态意义,其成员是世界各地森林的主要组成部分。在漫长的进化 "军备竞赛 "过程中,植物发展出了复杂多样的系统防御机制,从而有策略、有智慧地与食草动物竞争。为了研究食草动物取食刺激引发的变化模式和防御响应机制,我们首先建立了中国松(Pinus tabuliformis Carr.)与中国松毛虫(Dendrolimus tabulaeformis Tsai et Liu)之间相互关系的生物学模型。该模型整合了蛋白质组和磷蛋白组数据,然后将其归一化并与生物信息学工具相结合,以评估和分析磷蛋白组图谱在毛虫取食松针刺激下的变化。我们系统地鉴定了与松树对毛虫胁迫的防御机制有关的差异显著的磷酸化蛋白。此外,我们还预测了磷酸化位点的上游激酶及其活性。通过分析磷酸化蛋白的 Motif 模式、磷酸化位点的 Mfuzz 聚类和激酶调控网络,我们探索了松树应对胁迫的磷酸化蛋白相互作用网络的功能模块。总体而言,我们的研究首次强调了激酶 METK2、PTI12、PGK 以及 At3g59480 的重要作用。此外,平行反应监测技术也证实了这些磷酸化蛋白的鉴定。此外,还通过实时定量聚合酶链反应检测验证了与差异表达蛋白相关的基因。这项研究有助于了解松树抗性形成和毛虫取食诱因调控背后的机制。未来培育抗性更强的松树品种可能会受益于对这些防御策略更全面的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Defense response to caterpillar feeding stress in wild Pinus tabuliformis unveiled by quantitative integrated proteomic and phosphoproteomic analyses
Pinus is a genus of great economic and ecological importance, and its members are dominant components of forests throughout the world. During the long evolutionary “arms race,” plants have developed complex and diverse systemic defense mechanisms to strategically and intelligently compete with herbivores. To study the alteration pattern and defensive response mechanism triggered by herbivorous feeding stimuli, we firstly built a biological model of the interrelationship between the Chinese pine (Pinus tabuliformis Carr.) and the Chinese pine caterpillar (Dendrolimus tabulaeformis Tsai et Liu). This model integrated proteomic and phosphoproteomic data, which were then normalized and combined with bioinformatics tools to evaluate and analyze changes in the phosphoproteomic profile in response to the caterpillar’s feeding stimulus on pine needles. Systematic identification of differentially significant phosphorylated proteins implicated in the pine’s defense mechanism against caterpillar stress was conducted. Furthermore, we predicted upstream kinases of phosphorylation sites and their activities. Through an analysis of Motif patterns of phosphorylated proteins, Mfuzz clustering of phosphorylation sites, and kinase regulatory networks, we explored the functional modules of phosphorylated protein interaction networks in response to stress within pine. In general, our study emphasized the significant role of kinase METK2, PTI12, PGK, as well as At3g59480 for the first time. The identification of these phosphorylated proteins was additionally confirmed through parallel reaction monitoring technology. Furthermore, genes associated with differentially expressed proteins were validated through real-time quantitative polymerase chain reaction detection. This investigation aids in understanding the mechanisms behind resistance formation and regulation of caterpillar feeding incentives in pine. Breeding more resistant pine varieties may benefit from a fuller understanding of these defense strategies in the future.
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