{"title":"苓桂术甘汤对高脂饮食诱导的大鼠胰岛素抵抗模型的治疗作用","authors":"Xiao-Ming Liu, Shi-Qing Yuan, Ying-Yuan Ning, Shi-Jia Nie, Xu-Qiong Wang, Hong-Yi Jia, Xiu-Li Zheng","doi":"10.4239/wjd.v15.i6.1291","DOIUrl":null,"url":null,"abstract":"BACKGROUND\n Lingguizhugan (LGZG) decoction is a widely used classic Chinese medicine formula that was recently shown to improve high-fat diet (HFD)-induced insulin resistance (IR) in animal studies.\n AIM\n To assess the therapeutic effect of LGZG decoction on HFD-induced IR and explore the potential underlying mechanism.\n METHODS\n To establish an IR rat model, a 12-wk HFD was administered, followed by a 4-wk treatment with LGZG. The determination of IR status was achieved through the use of biochemical tests and oral glucose tolerance tests. Using a targeted meta-bolomics platform to analyze changes in serum metabolites, quantitative real-time PCR (qRT-PCR) was used to assess the gene expression of the ribosomal protein S6 kinase beta 1 (S6K1).\n RESULTS\n In IR rats, LGZG decreased body weight and indices of hepatic steatosis. It effectively controlled blood glucose and food intake while protecting islet cells. Metabolite analysis revealed significant differences between the HFD and HFD-LGZG groups. LGZG intervention reduced branched-chain amino acid levels. Levels of IR-related metabolites such as tryptophan, alanine, taurine, and asparagine decreased significantly. IR may be linked to amino acids due to the contemporaneous increase in S6K1 expression, as shown by qRT-PCR.\n CONCLUSIONS\n Our study strongly suggests that LGZG decoction reduces HFD-induced IR. LGZG may activate S6K1 via metabolic pathways. These findings lay the groundwork for the potential of LGZG as an IR treatment.","PeriodicalId":48607,"journal":{"name":"World Journal of Diabetes","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Therapeutic effects of Lingguizhugan decoction in a rat model of high-fat diet-induced insulin resistance\",\"authors\":\"Xiao-Ming Liu, Shi-Qing Yuan, Ying-Yuan Ning, Shi-Jia Nie, Xu-Qiong Wang, Hong-Yi Jia, Xiu-Li Zheng\",\"doi\":\"10.4239/wjd.v15.i6.1291\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"BACKGROUND\\n Lingguizhugan (LGZG) decoction is a widely used classic Chinese medicine formula that was recently shown to improve high-fat diet (HFD)-induced insulin resistance (IR) in animal studies.\\n AIM\\n To assess the therapeutic effect of LGZG decoction on HFD-induced IR and explore the potential underlying mechanism.\\n METHODS\\n To establish an IR rat model, a 12-wk HFD was administered, followed by a 4-wk treatment with LGZG. The determination of IR status was achieved through the use of biochemical tests and oral glucose tolerance tests. Using a targeted meta-bolomics platform to analyze changes in serum metabolites, quantitative real-time PCR (qRT-PCR) was used to assess the gene expression of the ribosomal protein S6 kinase beta 1 (S6K1).\\n RESULTS\\n In IR rats, LGZG decreased body weight and indices of hepatic steatosis. It effectively controlled blood glucose and food intake while protecting islet cells. Metabolite analysis revealed significant differences between the HFD and HFD-LGZG groups. LGZG intervention reduced branched-chain amino acid levels. Levels of IR-related metabolites such as tryptophan, alanine, taurine, and asparagine decreased significantly. IR may be linked to amino acids due to the contemporaneous increase in S6K1 expression, as shown by qRT-PCR.\\n CONCLUSIONS\\n Our study strongly suggests that LGZG decoction reduces HFD-induced IR. LGZG may activate S6K1 via metabolic pathways. These findings lay the groundwork for the potential of LGZG as an IR treatment.\",\"PeriodicalId\":48607,\"journal\":{\"name\":\"World Journal of Diabetes\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2024-06-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"World Journal of Diabetes\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.4239/wjd.v15.i6.1291\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"World Journal of Diabetes","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4239/wjd.v15.i6.1291","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
摘要
背景 灵桂术甘汤是一种广泛使用的经典中药配方,最近在动物实验中被证明可改善高脂饮食(HFD)诱导的胰岛素抵抗(IR)。目的 评估 LGZG 水煎剂对高脂饮食诱导的 IR 的治疗效果,并探索其潜在的内在机制。方法 为了建立 IR 大鼠模型,先给予 12 周高密度脂蛋白膳食,然后用 LGZG 治疗 4 周。通过生化测试和口服葡萄糖耐量测试来确定 IR 状态。使用靶向元生物组学平台分析血清代谢物的变化,使用定量实时 PCR (qRT-PCR) 评估核糖体蛋白 S6 激酶 beta 1 (S6K1) 的基因表达。结果 在 IR 大鼠中,LGZG 可降低体重和肝脏脂肪变性指数。它能有效控制血糖和食物摄入量,同时保护胰岛细胞。代谢物分析表明,HFD 组和 HFD-LGZG 组之间存在显著差异。LGZG干预降低了支链氨基酸水平。色氨酸、丙氨酸、牛磺酸和天冬酰胺等与IR相关的代谢物水平显著下降。正如 qRT-PCR 所示,由于 S6K1 的表达同时增加,IR 可能与氨基酸有关。结论 我们的研究有力地表明,LGZG 水煎剂可降低 HFD 诱导的 IR。LGZG 可通过代谢途径激活 S6K1。这些发现为 LGZG 治疗 IR 的潜力奠定了基础。
Therapeutic effects of Lingguizhugan decoction in a rat model of high-fat diet-induced insulin resistance
BACKGROUND
Lingguizhugan (LGZG) decoction is a widely used classic Chinese medicine formula that was recently shown to improve high-fat diet (HFD)-induced insulin resistance (IR) in animal studies.
AIM
To assess the therapeutic effect of LGZG decoction on HFD-induced IR and explore the potential underlying mechanism.
METHODS
To establish an IR rat model, a 12-wk HFD was administered, followed by a 4-wk treatment with LGZG. The determination of IR status was achieved through the use of biochemical tests and oral glucose tolerance tests. Using a targeted meta-bolomics platform to analyze changes in serum metabolites, quantitative real-time PCR (qRT-PCR) was used to assess the gene expression of the ribosomal protein S6 kinase beta 1 (S6K1).
RESULTS
In IR rats, LGZG decreased body weight and indices of hepatic steatosis. It effectively controlled blood glucose and food intake while protecting islet cells. Metabolite analysis revealed significant differences between the HFD and HFD-LGZG groups. LGZG intervention reduced branched-chain amino acid levels. Levels of IR-related metabolites such as tryptophan, alanine, taurine, and asparagine decreased significantly. IR may be linked to amino acids due to the contemporaneous increase in S6K1 expression, as shown by qRT-PCR.
CONCLUSIONS
Our study strongly suggests that LGZG decoction reduces HFD-induced IR. LGZG may activate S6K1 via metabolic pathways. These findings lay the groundwork for the potential of LGZG as an IR treatment.
期刊介绍:
The WJD is a high-quality, peer reviewed, open-access journal. The primary task of WJD is to rapidly publish high-quality original articles, reviews, editorials, and case reports in the field of diabetes. In order to promote productive academic communication, the peer review process for the WJD is transparent; to this end, all published manuscripts are accompanied by the anonymized reviewers’ comments as well as the authors’ responses. The primary aims of the WJD are to improve diagnostic, therapeutic and preventive modalities and the skills of clinicians and to guide clinical practice in diabetes. Scope: Diabetes Complications, Experimental Diabetes Mellitus, Type 1 Diabetes Mellitus, Type 2 Diabetes Mellitus, Diabetes, Gestational, Diabetic Angiopathies, Diabetic Cardiomyopathies, Diabetic Coma, Diabetic Ketoacidosis, Diabetic Nephropathies, Diabetic Neuropathies, Donohue Syndrome, Fetal Macrosomia, and Prediabetic State.