富者愈富 "的机制推动了抗生素处理过的细菌的斑块动态和耐药性进化。

IF 8.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular Systems Biology Pub Date : 2024-08-01 Epub Date: 2024-06-14 DOI:10.1038/s44320-024-00046-5
Emrah Şimşek, Kyeri Kim, Jia Lu, Anita Silver, Nan Luo, Charlotte T Lee, Lingchong You
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引用次数: 0

摘要

自然界中的细菌通常会形成表面附着群落,最初由不同的亚群或斑块组成。对于病原体来说,这些斑块可以在感染部位形成,在抗生素治疗期间持续存在,并发展成成熟的生物膜。有证据表明,斑块的出现可能是由于生长环境和细菌播种的异质性以及细胞-细胞信号传递。然而,目前还不清楚这些因素如何促成斑块的形成,以及斑块的形成如何影响细菌的生存和进化。在这里,我们证明了一种 "富者愈富 "的机制会在抗生素治疗期间驱动表现出集体生存(CS)的细菌形成斑块。根据建模预测,这些细菌的播种异质性会因局部 CS 和全局资源竞争而放大,从而导致斑块形成。增加非消除性抗生素治疗的剂量会增加斑块的程度。在实验中,我们首先利用工程大肠杆菌证明了这一机制,然后证明了它对病原体铜绿假单胞菌的适用性。我们进一步证明,铜绿假单胞菌斑块的形成促进了抗生素耐药性的进化。我们的研究为了解表面附着细菌生长过程中的种群动态和耐药性进化提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A 'rich-get-richer' mechanism drives patchy dynamics and resistance evolution in antibiotic-treated bacteria.

Bacteria in nature often form surface-attached communities that initially comprise distinct subpopulations, or patches. For pathogens, these patches can form at infection sites, persist during antibiotic treatment, and develop into mature biofilms. Evidence suggests that patches can emerge due to heterogeneity in the growth environment and bacterial seeding, as well as cell-cell signaling. However, it is unclear how these factors contribute to patch formation and how patch formation might affect bacterial survival and evolution. Here, we demonstrate that a 'rich-get-richer' mechanism drives patch formation in bacteria exhibiting collective survival (CS) during antibiotic treatment. Modeling predicts that the seeding heterogeneity of these bacteria is amplified by local CS and global resource competition, leading to patch formation. Increasing the dose of a non-eradicating antibiotic treatment increases the degree of patchiness. Experimentally, we first demonstrated the mechanism using engineered Escherichia coli and then demonstrated its applicability to a pathogen, Pseudomonas aeruginosa. We further showed that the formation of P. aeruginosa patches promoted the evolution of antibiotic resistance. Our work provides new insights into population dynamics and resistance evolution during surface-attached bacterial growth.

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来源期刊
Molecular Systems Biology
Molecular Systems Biology 生物-生化与分子生物学
CiteScore
18.50
自引率
1.00%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Systems biology is a field that aims to understand complex biological systems by studying their components and how they interact. It is an integrative discipline that seeks to explain the properties and behavior of these systems. Molecular Systems Biology is a scholarly journal that publishes top-notch research in the areas of systems biology, synthetic biology, and systems medicine. It is an open access journal, meaning that its content is freely available to readers, and it is peer-reviewed to ensure the quality of the published work.
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