补充褪黑素对稻田鳗鲡生长性能和肠道健康的影响

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Weiwei Lv , Muyan Li , Yifan Mao , Weiwei Huang , Quan Yuan , Mingyou Li , Qiubai Zhou , Hang Yang , Wenzong Zhou
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引用次数: 0

摘要

目的是评估补充褪黑激素对稻田鳗鲡生长性能和肠道健康的影响。研究人员用 0、30、60、120 和 240 毫克/千克的褪黑激素添加剂喂养了 360 尾鱼(28.46 ± 0.24 克),连续喂养 70 天。研究发现,FBW、WGR、SGR 和 FCR 变量与日粮褪黑素的关系呈显著的二次方统计关系(P < 0.05),120 毫克/千克褪黑激素组的 FBW、WGR 和 SGR 最高,FCR 最低;消化酶活性(如淀粉酶、胰蛋白酶和脂肪酶)与日粮褪黑激素浓度也呈显著的二次方关系(P < 0.05),120 毫克/千克日粮中的肠绒毛高度和鹅口疮细胞最高(P < 0.01),日粮中褪黑素能显著提高血清中SOD和CAT活性,上调抗炎因子(IL-10)和紧密连接蛋白(ZO-1)的表达,下调促炎因子(IL-1β、IL-8、IL-15、膳食褪黑素改善了肠道微生物区系的组成,与对照组相比,补充 120 毫克/千克膳食褪黑素的组中,肠道固缩菌的丰度和固缩菌/类杆菌的比例明显上升(P <;0.1).因此,日粮添加褪黑素可促进白鲑鱼的生长性能,提高肠道免疫能力和血清抗氧化剂水平,改善肠道形态特性和肠道菌群组成。总之,根据 WGR 和 FCR 的二次折线回归分析,预测褪黑素的最佳供给浓度为 146-148 mg/kg。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of dietary melatonin supplementation on growth performance and intestinal health of rice field eel (Monopterus albus)

Effects of dietary melatonin supplementation on growth performance and intestinal health of rice field eel (Monopterus albus)

The objective was to assess the impact of melatonin supplementation on the growth performance and intestinal health of rice field eel, Monopterus albus. Three hundred and sixty fish (28.46 ± 0.24 g) were fed five diets supplemented with melatonin of 0, 30, 60, 120, and 240 mg/kg for 70 days. The study found that the variables FBW, WGR, SGR, and FCR exhibited a statistically significant quadratic relationship (P < 0.05) with the dietary melatonin concentrations, and the highest FBW, WGR and SGR as well as lowest FCR were observed in the 120 mg/kg melatonin group, digestive enzymes activities (such as amylase, trypsin, and lipase) also had significant quadratic relationship (P < 0.05), and the highest intestinal villus height and goblet cells were found in the 120 mg/kg diet (P < 0.01), melatonin in diets significantly increased SOD and CAT activities in serum, up-regulated the expression of anti-inflammatory factors (IL-10) and tight junction protein (ZO-1), and down-regulated the expression of pro-inflammatory factors (IL-1β, IL-8, IL-15, and TNF-α) in the gut, dietary melatonin improved the intestinal microflora compositions, in the group that supplementation a dosage of 120 mg/kg, there was a noticeable rise in the abundance of Firmicutes and the ratio of Firmicutes/Bacteroidota, compared with control group (P < 0.1). Conclusively, dietary supplementation of melatonin promoted growth performance, enhanced intestinal immune capacity and serum antioxidant level, and improved intestinal morphology properties and intestinal flora composition in M. albus. In conclusion, based on quadratic broken-line regression analysis of WGR and FCR, the optimal concentration of melatonin to be supplied is predicted to be 146–148 mg/kg.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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