香叶醇对大鼠肝缺血再灌注损伤模型的作用

Emre Tunç, Vedat Durgun, Ozan Akıncı, Sefa Ergün, Osman Şimşek, Ibrahim Murat Bolayırlı, Nuray Kepil
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引用次数: 0

摘要

背景:肝脏缺血/再灌注(I/R)损伤是一种严重的临床症状,可在肝脏切除、创伤和休克时发生。香叶醇是一种常见于自然界的异萜分子,具有抗氧化和保护肝脏的特性。本研究通过诱导大鼠实验性肝脏 I/R 损伤,探讨了香叶醇对肝损伤的影响:本研究使用了 28 只体重 350-400 克的雄性 Wistar Albino 大鼠。大鼠分为四组:对照组、I/R 组、50 毫克/千克香叶醇+I/R 组和 100 毫克/千克香叶醇+I/R 组。缺血时间定为 15 分钟,再灌注时间定为 20 分钟。缺血从服用香叶醇 15 分钟后开始。检测血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和乳酸的水平,以及肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)的活性水平。还对肝组织进行了组织病理学检查:结果:腹腔注射 50 毫克/千克和 100 毫克/千克香叶醇可显著降低谷草转氨酶(AST)、乳酸和肿瘤坏死因子-α(TNF-α)的水平。50 毫克/千克组的血清谷丙转氨酶水平明显下降,而 100 毫克/千克组没有明显下降。100 毫克/千克组的 SOD 和 GPx 酶活性明显增加。虽然 50 毫克/千克组中这些酶的含量有所增加,但在统计学上并不显著。同样,CAT 酶活性在 50 毫克/千克组和 100 毫克/千克组中都有所增加,但增幅不明显。50 毫克/千克组和 100 毫克/千克组的铃木评分均明显下降:研究表明,香叶醇可减少肝脏生化和组织病理学损伤,并增加抗氧化防御酶。这些研究结果表明,如果得到大规模综合研究的证实,香叶醇可用于预防肝脏 I/R 损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of geraniol on hepatic ischemia-reperfusion injury model in rats.

Background: Hepatic ischemia/reperfusion (I/R) injury is a significant clinical condition that can arise during liver resections, trauma, and shock. Geraniol, an isoterpene molecule commonly found in nature, possesses antioxidant and hepatoprotective properties. This study investigates the impact of geraniol on hepatic damage by inducing experimental liver I/R injury in rats.

Methods: Twenty-eight male Wistar Albino rats weighing 350-400 g were utilized for this study. The rats were divided into four groups: control group, I/R group, 50 mg/kg geraniol+I/R group, and 100 mg/kg geraniol+I/R group. Ischemia times were set at 15 minutes with reperfusion times at 20 minutes. Ischemia commenced 15 minutes after geraniol administration. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactic acid were measured, along with superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity levels in liver tissues. Liver tissues were also examined histopathologically.

Results: It was observed that intraperitoneal administration of 50 mg/kg and 100 mg/kg geraniol significantly reduced AST, lactic acid, and tumor necrosis factor-alpha (TNF-α) levels. The serum ALT level decreased significantly in the 50 mg/kg group, whereas no significant decrease was found in the 100 mg/kg group. SOD and GPx enzyme activities were shown to increase significantly in the 100 mg/kg group. Although there was an increase in these enzyme levels in the 50 mg/kg group, it was not statistically significant. Similarly, CAT enzyme activity increased in both the 50 mg/kg and 100 mg/kg groups, but the increase was not significant. The Suzuki score significantly decreased in both the 50 mg/kg and 100 mg/kg groups.

Conclusion: The study demonstrates that geraniol reduced hepatic damage both biochemically and histopathologically and increased antioxidant defense enzymes. These findings suggest that geraniol could be used to prevent hepatic I/R injury, provided it is corroborated by large-scale and comprehensive studies.

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