{"title":"在大肠杆菌中开发由法定量感应介导的细菌自溶系统,以自动释放胞内产物。","authors":"Xiaofei Song, Yifan Zhao, Yixuan Ren, Ruoyu Liu, Mengting Zhang, Zhikai Zhang, Qiu Meng, Tingheng Zhu, Jianhua Yin and Zhiliang Yu*, ","doi":"10.1021/acssynbio.4c00084","DOIUrl":null,"url":null,"abstract":"<p ><i>Escherichia coli</i>, one of the most efficient expression hosts for recombinant proteins, is widely used in chemical, medical, food, and other industries. <i>De novo</i> engineering of gene regulation circuits and cell density-controlled <i>E. coli</i> cell lysis are promising directions for the release of intracellular bioproducts. Here, we developed an <i>E. coli</i> autolytic system, named the quorum sensing-mediated bacterial autolytic (QS-BA) system, by incorporating an acyl-homoserine lactone (AHL)-based YasI/YasR-type quorum sensing circuit from <i>Pseudoalteromonas</i> into <i>E. coli</i> cells. The results showed that the <i>E. coli</i> QS-BA system can release the intracellular bioproducts into the cell culture medium in terms of <i>E. coli</i> cell density, which offers an environmentally-friendly, economical, efficient, and flexible <i>E. coli</i> lysis platform for production of recombinant proteins. The QS-BA system has the potential to serve as an integrated system for the large-scale production of target products in <i>E. coli</i> for medical and industrial applications.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of a Quorum Sensing-Mediated Bacterial Autolytic System in Escherichia coli for Automatic Release of Intracellular Products\",\"authors\":\"Xiaofei Song, Yifan Zhao, Yixuan Ren, Ruoyu Liu, Mengting Zhang, Zhikai Zhang, Qiu Meng, Tingheng Zhu, Jianhua Yin and Zhiliang Yu*, \",\"doi\":\"10.1021/acssynbio.4c00084\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p ><i>Escherichia coli</i>, one of the most efficient expression hosts for recombinant proteins, is widely used in chemical, medical, food, and other industries. <i>De novo</i> engineering of gene regulation circuits and cell density-controlled <i>E. coli</i> cell lysis are promising directions for the release of intracellular bioproducts. Here, we developed an <i>E. coli</i> autolytic system, named the quorum sensing-mediated bacterial autolytic (QS-BA) system, by incorporating an acyl-homoserine lactone (AHL)-based YasI/YasR-type quorum sensing circuit from <i>Pseudoalteromonas</i> into <i>E. coli</i> cells. The results showed that the <i>E. coli</i> QS-BA system can release the intracellular bioproducts into the cell culture medium in terms of <i>E. coli</i> cell density, which offers an environmentally-friendly, economical, efficient, and flexible <i>E. coli</i> lysis platform for production of recombinant proteins. The QS-BA system has the potential to serve as an integrated system for the large-scale production of target products in <i>E. coli</i> for medical and industrial applications.</p>\",\"PeriodicalId\":26,\"journal\":{\"name\":\"ACS Synthetic Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Synthetic Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acssynbio.4c00084\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Synthetic Biology","FirstCategoryId":"99","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acssynbio.4c00084","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Development of a Quorum Sensing-Mediated Bacterial Autolytic System in Escherichia coli for Automatic Release of Intracellular Products
Escherichia coli, one of the most efficient expression hosts for recombinant proteins, is widely used in chemical, medical, food, and other industries. De novo engineering of gene regulation circuits and cell density-controlled E. coli cell lysis are promising directions for the release of intracellular bioproducts. Here, we developed an E. coli autolytic system, named the quorum sensing-mediated bacterial autolytic (QS-BA) system, by incorporating an acyl-homoserine lactone (AHL)-based YasI/YasR-type quorum sensing circuit from Pseudoalteromonas into E. coli cells. The results showed that the E. coli QS-BA system can release the intracellular bioproducts into the cell culture medium in terms of E. coli cell density, which offers an environmentally-friendly, economical, efficient, and flexible E. coli lysis platform for production of recombinant proteins. The QS-BA system has the potential to serve as an integrated system for the large-scale production of target products in E. coli for medical and industrial applications.
期刊介绍:
The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism.
Topics may include, but are not limited to:
Design and optimization of genetic systems
Genetic circuit design and their principles for their organization into programs
Computational methods to aid the design of genetic systems
Experimental methods to quantify genetic parts, circuits, and metabolic fluxes
Genetic parts libraries: their creation, analysis, and ontological representation
Protein engineering including computational design
Metabolic engineering and cellular manufacturing, including biomass conversion
Natural product access, engineering, and production
Creative and innovative applications of cellular programming
Medical applications, tissue engineering, and the programming of therapeutic cells
Minimal cell design and construction
Genomics and genome replacement strategies
Viral engineering
Automated and robotic assembly platforms for synthetic biology
DNA synthesis methodologies
Metagenomics and synthetic metagenomic analysis
Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction
Gene optimization
Methods for genome-scale measurements of transcription and metabolomics
Systems biology and methods to integrate multiple data sources
in vitro and cell-free synthetic biology and molecular programming
Nucleic acid engineering.