Chunyan Li , Xinru Zhang , Maolin Wang , Zhu Wang , Qi Cheng , Junrong Du , Tao Jia
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引用次数: 0
摘要
肺泡是呼吸系统中最小的结构和功能单元,在维持肺功能方面发挥着至关重要的作用。肺泡损伤是呼吸系统疾病的典型病理标志。然而,目前还没有一种简单、快速、经济、无偏见的方法来量化整个肺组织的肺泡大小。在此,我们首先根据不同肺叶气道分支的大小、形状和分布情况对肺样本进行切片。接着,我们对不同切片进行 HE 染色。然后,我们使用免费软件 ImageJ 对肺泡大小进行了无偏量化。通过这一方案,我们证明了 C57Bl/6 小鼠不同肺叶的肺泡大小不同。总之,我们为更全面地量化小鼠肺泡大小提供了一种简单而无偏见的方法,这为利用小鼠模型进行更广泛的呼吸系统研究带来了希望。
A simple and economical method for unbiasedly quantifying the alveolar size of entire mouse lung tissue utilizing Hematoxylin and Eosin Staining
Alveolar, the smallest structural and functional units within the respiratory system, play a crucial role in maintaining lung function. Alveolar damage is a typical pathological hallmark of respiratory diseases. Nevertheless, there is currently no simple, rapid, economical, and unbiased method for quantifying alveolar size for entire lung tissue. Here, firstly, we conducted lung sample slicing based on the size, shape, and distribution of airway branches of different lobes. Next, we performed HE staining on different slices. Then, we provided an unbiased quantification of alveolar size using free software ImageJ. Through this protocol, we demonstrated that C57Bl/6 mice exhibit varying alveolar sizes among different lobes. Collectively, we provided a simple and unbiased method for a more comprehensive quantification of alveolar size in mice, which holds promise for a broader range of respiratory research using mouse models.
期刊介绍:
Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted