父母缺锌对黑腹果蝇代谢和氧化还原结果的代际影响

Biology Pub Date : 2024-06-01 DOI:10.3390/biology13060401
K. Sanusi, K. Ibrahim, M. Abubakar, T. S. Shinkafi, Aminu Ishaka, M. Imam
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引用次数: 0

摘要

缺锌是一种常见的营养失调症,会对健康造成不利影响。父母缺锌是否会诱发代际效应仍是一个未知数。我们研究了母代和父代锌缺乏对黑腹果蝇后代代谢结果和基因表达变化的影响。亲代果蝇在整个发育过程中都以缺锌饮食饲养,并对其后代进行评估。缺锌亲本的后代体重和全身锌水平显著增加(p < 0.05)。它们还表现出葡萄糖代谢紊乱、脂质平衡改变和抗氧化酶活性降低。基因表达分析表明,锌转运基因发生了显著变化(p < 0.05),雌性和雄性后代的 dZIP1 和 dZnT1 的 mRNA 水平分别升高。雌雄后代的dZnT35C mRNA水平都有所降低,DILP2和促炎症标记物Eiger和UPD2的mRNA水平显著升高(p < 0.05)。总体而言,雌性后代对父母缺锌的敏感性更高。我们的研究结果强调了锌在维持健康方面的重要作用,以及锌缺乏时的性别特异性反应。我们需要进一步探索这些代际效应背后的潜在机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intergenerational Impact of Parental Zinc Deficiency on Metabolic and Redox Outcomes in Drosophila melanogaster
Zinc deficiency is a common nutritional disorder with detrimental health consequences. Whether parental zinc deficiency induces intergenerational effects remains largely unknown. We investigated the effects of a combined maternal and paternal zinc deficiency on offspring’s metabolic outcomes and gene expression changes in Drosophila melanogaster. The parent flies were raised on zinc-deficient diets throughout development, and their progeny were assessed. Offspring from zinc-deprived parents exhibited a significant (p < 0.05) increase in body weight and whole-body zinc levels. They also displayed disrupted glucose metabolism, altered lipid homeostasis, and diminished activity of antioxidant enzymes. Gene expression analysis revealed significant (p < 0.05) alterations in zinc transport genes, with increases in mRNA levels of dZIP1 and dZnT1 for female and male offspring, respectively. Both sexes exhibited reduced dZnT35C mRNA levels and significant (p < 0.05) increases in the mRNA levels of DILP2 and proinflammatory markers, Eiger and UPD2. Overall, female offspring showed higher sensitivity to parental zinc deficiency. Our findings underscore zinc’s crucial role in maintaining health and the gender-specific responses to zinc deficiency. There is the need for further exploration of the underlying mechanisms behind these intergenerational effects.
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