经菌根真菌和谷胱甘肽处理的油茶籽脂肪酸谱的变化

IF 3.1 3区 农林科学 Q1 HORTICULTURE
Wei-Jia Wu, Ying-Ning Zou, Zhiyan Xiao, Fang-Ling Wang, A. Hashem, E. F. Abd_Allah, Qiangsheng Wu
{"title":"经菌根真菌和谷胱甘肽处理的油茶籽脂肪酸谱的变化","authors":"Wei-Jia Wu, Ying-Ning Zou, Zhiyan Xiao, Fang-Ling Wang, A. Hashem, E. F. Abd_Allah, Qiangsheng Wu","doi":"10.3390/horticulturae10060580","DOIUrl":null,"url":null,"abstract":"Camellia oleifera is an important oilseed forest tree, but it is unknown whether and how inoculation with arbuscular mycorrhizal fungi, as well as spraying easily extractable glomalin-related soil protein (EG), regulates the fatty acid profile in seeds of this species. This study explored how inoculation with Rhizophagus intraradices (800 g inoculum/tree) and spraying EG (2.5 L/tree, four times in total, once a week) modulated the fatty acid profile for potential nutritional qualities in the seeds of 20-year-old C. oleifera. Spraying exogenous EG significantly increased fruit transverse diameter, longitudinal diameter, fruit weight, number of seeds, and seed weight but had no significant effect on the root mycorrhizal colonization rate. Inoculation with R. intraradices had no significant effect on these fruit traits but significantly boosted the root mycorrhizal colonization rate. A total of 11 saturated fatty acids and 12 unsaturated fatty acids were detected from the seeds, with the unsaturated fatty acids consisting primarily of C18:1N-12, C18:1N-9C, and C18:2-N6. Spraying exogenous EG significantly increased the levels of major unsaturated fatty acid components such as C18:1N-12, C18:1N-9C, C18:1N-7, and C18:2N-6 by 140.6%, 59.7%, 97.6%, and 60.6%, respectively, while decreasing the level of C16:0. Inoculation with R. intraradices only decreased the levels of C16:0 and C18:0, while increased the level of C18:2N-6. Both treatments increased the percentage of unsaturated fatty acids in total fatty acids, resulting in an increase in the unsaturation index of fatty acids. In addition, inoculation with R. intraradices significantly up-regulated the expression of CoFAD2, spraying exogenous EG significantly increased the expression of CoSAD, CoFAD2, and CoFAD3, and both treatments also significantly suppressed the expression of CoFAE. These findings suggested that exogenous EG as a biostimulant, is more suitable to regulate the nutritional values of fatty acids in seeds of 20-year-old C. oleifera.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Changes in Fatty Acid Profiles in Seeds of Camellia oleifera Treated by Mycorrhizal Fungi and Glomalin\",\"authors\":\"Wei-Jia Wu, Ying-Ning Zou, Zhiyan Xiao, Fang-Ling Wang, A. Hashem, E. F. Abd_Allah, Qiangsheng Wu\",\"doi\":\"10.3390/horticulturae10060580\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Camellia oleifera is an important oilseed forest tree, but it is unknown whether and how inoculation with arbuscular mycorrhizal fungi, as well as spraying easily extractable glomalin-related soil protein (EG), regulates the fatty acid profile in seeds of this species. This study explored how inoculation with Rhizophagus intraradices (800 g inoculum/tree) and spraying EG (2.5 L/tree, four times in total, once a week) modulated the fatty acid profile for potential nutritional qualities in the seeds of 20-year-old C. oleifera. Spraying exogenous EG significantly increased fruit transverse diameter, longitudinal diameter, fruit weight, number of seeds, and seed weight but had no significant effect on the root mycorrhizal colonization rate. Inoculation with R. intraradices had no significant effect on these fruit traits but significantly boosted the root mycorrhizal colonization rate. A total of 11 saturated fatty acids and 12 unsaturated fatty acids were detected from the seeds, with the unsaturated fatty acids consisting primarily of C18:1N-12, C18:1N-9C, and C18:2-N6. Spraying exogenous EG significantly increased the levels of major unsaturated fatty acid components such as C18:1N-12, C18:1N-9C, C18:1N-7, and C18:2N-6 by 140.6%, 59.7%, 97.6%, and 60.6%, respectively, while decreasing the level of C16:0. Inoculation with R. intraradices only decreased the levels of C16:0 and C18:0, while increased the level of C18:2N-6. Both treatments increased the percentage of unsaturated fatty acids in total fatty acids, resulting in an increase in the unsaturation index of fatty acids. In addition, inoculation with R. intraradices significantly up-regulated the expression of CoFAD2, spraying exogenous EG significantly increased the expression of CoSAD, CoFAD2, and CoFAD3, and both treatments also significantly suppressed the expression of CoFAE. These findings suggested that exogenous EG as a biostimulant, is more suitable to regulate the nutritional values of fatty acids in seeds of 20-year-old C. oleifera.\",\"PeriodicalId\":13034,\"journal\":{\"name\":\"Horticulturae\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-06-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3390/horticulturae10060580\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/horticulturae10060580","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0

摘要

油茶(Camellia oleifera)是一种重要的油料林树种,但接种丛枝菌根真菌以及喷洒易提取的胶霉素相关土壤蛋白质(EG)是否以及如何调节该树种种子的脂肪酸组成尚不清楚。本研究探讨了接种根瘤菌(800 克接种体/棵树)和喷洒 EG(2.5 升/棵树,共四次,每周一次)如何调节油橄榄 20 年树龄种子的脂肪酸组成,以获得潜在的营养品质。喷洒外源 EG 能显著增加果实横径、纵径、果实重量、种子数量和种子重量,但对根菌根定殖率没有显著影响。接种 R. intraradices 对这些果实性状没有明显影响,但对根菌根的定殖率有明显提高。从种子中总共检测到 11 种饱和脂肪酸和 12 种不饱和脂肪酸,不饱和脂肪酸主要包括 C18:1N-12、C18:1N-9C 和 C18:2-N6。喷洒外源 EG 能显著提高 C18:1N-12、C18:1N-9C、C18:1N-7 和 C18:2N-6 等主要不饱和脂肪酸成分的含量,增幅分别为 140.6%、59.7%、97.6% 和 60.6%,同时降低 C16:0 的含量。接种 R. intraradices 只降低了 C16:0 和 C18:0 的含量,而提高了 C18:2N-6 的含量。这两种处理都增加了不饱和脂肪酸在总脂肪酸中所占的比例,导致脂肪酸的不饱和指数增加。此外,接种 R. intraradices 能显著上调 CoFAD2 的表达,喷洒外源 EG 能显著提高 CoSAD、CoFAD2 和 CoFAD3 的表达,这两种处理还能显著抑制 CoFAE 的表达。这些研究结果表明,外源 EG 作为一种生物刺激剂,更适于调节 20 年油茶籽中脂肪酸的营养价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Changes in Fatty Acid Profiles in Seeds of Camellia oleifera Treated by Mycorrhizal Fungi and Glomalin
Camellia oleifera is an important oilseed forest tree, but it is unknown whether and how inoculation with arbuscular mycorrhizal fungi, as well as spraying easily extractable glomalin-related soil protein (EG), regulates the fatty acid profile in seeds of this species. This study explored how inoculation with Rhizophagus intraradices (800 g inoculum/tree) and spraying EG (2.5 L/tree, four times in total, once a week) modulated the fatty acid profile for potential nutritional qualities in the seeds of 20-year-old C. oleifera. Spraying exogenous EG significantly increased fruit transverse diameter, longitudinal diameter, fruit weight, number of seeds, and seed weight but had no significant effect on the root mycorrhizal colonization rate. Inoculation with R. intraradices had no significant effect on these fruit traits but significantly boosted the root mycorrhizal colonization rate. A total of 11 saturated fatty acids and 12 unsaturated fatty acids were detected from the seeds, with the unsaturated fatty acids consisting primarily of C18:1N-12, C18:1N-9C, and C18:2-N6. Spraying exogenous EG significantly increased the levels of major unsaturated fatty acid components such as C18:1N-12, C18:1N-9C, C18:1N-7, and C18:2N-6 by 140.6%, 59.7%, 97.6%, and 60.6%, respectively, while decreasing the level of C16:0. Inoculation with R. intraradices only decreased the levels of C16:0 and C18:0, while increased the level of C18:2N-6. Both treatments increased the percentage of unsaturated fatty acids in total fatty acids, resulting in an increase in the unsaturation index of fatty acids. In addition, inoculation with R. intraradices significantly up-regulated the expression of CoFAD2, spraying exogenous EG significantly increased the expression of CoSAD, CoFAD2, and CoFAD3, and both treatments also significantly suppressed the expression of CoFAE. These findings suggested that exogenous EG as a biostimulant, is more suitable to regulate the nutritional values of fatty acids in seeds of 20-year-old C. oleifera.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Horticulturae
Horticulturae HORTICULTURE-
CiteScore
3.50
自引率
19.40%
发文量
998
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信