塞马鲁肽可改善宫内粘连模型中的纤维化并抑制上皮-间质转化

Luming Wu, Yue Zhan, Yiqing Wang
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摘要

本研究的目的是探讨塞马鲁肽对宫腔内粘连的影响,并发现治疗宫腔内粘连的新药物。本研究以TGF-β1诱导的人子宫内膜上皮细胞模拟细胞模型,通过机械刮宫和炎症刺激建立动物模型。在添加或不添加不同浓度的塞马鲁肽与TGF-β1共同培养48小时后,收集细胞进行RT-qPCR和Western印迹分析。实验组小鼠皮下注射三个剂量的塞马鲁肽,每天一次,连续两周;对照组小鼠注射无菌 ddH2O。收集小鼠血清和子宫组织。采用 HE 和 Masson 染色法进行子宫组织形态学和病理学分析。RT-qPCR和Western印迹法用于mRNA和蛋白质表达分析。使用 ELISA 试剂盒检测血清指标。结果显示,塞马鲁肽能显著降低两种模型中纤维化指标ACTA2、COL1A1和FN以及炎症指标TNF-α、IL-6和NF-κB的mRNA水平。塞马鲁肽改善了IUA小鼠的子宫内膜形态,增加了子宫内膜腺体的数量,并减少了胶原沉积。结果还显示,塞马鲁肽能抑制两种模型中的波形蛋白、E-Cadherin和N-Cadherin。总之,塞马鲁肽可以改善宫腔内粘连的纤维化和炎症,并抑制IUA模型的上皮-间质转化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Semaglutide May Ameliorate Fibrosis and Inhibit Epithelial–Mesenchymal Transition in Intrauterine Adhesion Models
The purpose of this study was to explore the effect of Semaglutide on intrauterine adhesions and discover new drugs for such adhesions. In this study, the cell model was simulated by TGF-β1-induced human endometrial epithelial cells, and the animal model was established through mechanical curettage and inflammatory stimulation. After co-culturing with TGF-β1 with or without different concentrations of Semaglutide for 48 h, cells were collected for RT-qPCR and Western blotting analyses. Three doses were subcutaneously injected into experimental mice once a day for two weeks, while the control group received sterile ddH2O. The serum and uterine tissues of the mice were collected. HE and Masson staining were used for the uterine histomorphological and pathological analyses. RT-qPCR and Western blotting were used for mRNA and protein expression analyses. Serum indicators were detected using ELISA kits. The results showed that Semaglutide significantly reduced the mRNA levels of fibrosis indicators ACTA2, COL1A1, and FN and inflammatory indicators TNF-α, IL-6, and NF-κB in the two models. Semaglutide improved endometrium morphology, increased the number of endometrial glands, and reduced collagen deposition in IUA mice. The results also showed that Semaglutide could inhibit vimentin, E-Cadherin, and N-Cadherin in the two models. In summary, Semaglutide can ameliorate fibrosis and inflammation of intrauterine adhesions as well as inhibit epithelial–mesenchymal transition in IUA models.
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