鸡肝脏脂肪变性的机制:宿主基因组、分子表型组学和肠道微生物组的综合分析。

IF 11.8 2区 生物学 Q1 MULTIDISCIPLINARY SCIENCES
Congjiao Sun, Fangren Lan, Qianqian Zhou, Xiaoli Guo, Jiaming Jin, Chaoliang Wen, Yanxin Guo, Zhuocheng Hou, Jiangxia Zheng, Guiqin Wu, Guangqi Li, Yiyuan Yan, Junying Li, Qiugang Ma, Ning Yang
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引用次数: 0

摘要

肝脏脂肪变性是肝功能异常的最初表现,通常会导致肝脏疾病,如人类的非酒精性脂肪肝和动物的脂肪肝综合征。在这项研究中,我们对一个由 705 只成年母鸡组成的大型鸡群体进行了综合分析,结合了宿主基因组重测序;肝脏转录组、蛋白质组和代谢组分析;以及肠道各段微生物 16S 核糖体 RNA 基因测序。结果表明,肝脂肪变性的遗传率(h2 = 0.25)和十二指肠微生物率(m2 = 0.26)相对较高,表明宿主遗传和十二指肠微生物群对鸡肝脂肪变性有很大影响。肝脂肪变性个体的微生物群多样性较低,处理肝细胞甘油三酯输出的遗传潜力、脂肪酸β-氧化活性和抵抗脂肪酸过氧化的能力下降。此外,我们还发现了一个连接宿主基因组变异(GGA6:5.59-5.69Mb)、肝脏基因/蛋白表达(PEMT、磷脂酰乙醇胺 N-甲基转移酶)、代谢物丰度(叶酸、S-腺苷蛋氨酸、同型半胱氨酸、磷脂酰乙醇胺和磷脂酰胆碱)以及十二指肠微生物(乳酸杆菌属)与肝脏脂肪变性之间的分子网络,这可能为脂肪肝发生的调控机制提供新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mechanisms of hepatic steatosis in chickens: integrated analysis of the host genome, molecular phenomics and gut microbiome.

Hepatic steatosis is the initial manifestation of abnormal liver functions and often leads to liver diseases such as nonalcoholic fatty liver disease in humans and fatty liver syndrome in animals. In this study, we conducted a comprehensive analysis of a large chicken population consisting of 705 adult hens by combining host genome resequencing; liver transcriptome, proteome, and metabolome analysis; and microbial 16S ribosomal RNA gene sequencing of each gut segment. The results showed the heritability (h2 = 0.25) and duodenal microbiability (m2 = 0.26) of hepatic steatosis were relatively high, indicating a large effect of host genetics and duodenal microbiota on chicken hepatic steatosis. Individuals with hepatic steatosis had low microbiota diversity and a decreased genetic potential to process triglyceride output from hepatocytes, fatty acid β-oxidation activity, and resistance to fatty acid peroxidation. Furthermore, we revealed a molecular network linking host genomic variants (GGA6: 5.59-5.69 Mb), hepatic gene/protein expression (PEMT, phosphatidyl-ethanolamine N-methyltransferase), metabolite abundances (folate, S-adenosylmethionine, homocysteine, phosphatidyl-ethanolamine, and phosphatidylcholine), and duodenal microbes (genus Lactobacillus) to hepatic steatosis, which could provide new insights into the regulatory mechanism of fatty liver development.

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来源期刊
GigaScience
GigaScience MULTIDISCIPLINARY SCIENCES-
CiteScore
15.50
自引率
1.10%
发文量
119
审稿时长
1 weeks
期刊介绍: GigaScience seeks to transform data dissemination and utilization in the life and biomedical sciences. As an online open-access open-data journal, it specializes in publishing "big-data" studies encompassing various fields. Its scope includes not only "omic" type data and the fields of high-throughput biology currently serviced by large public repositories, but also the growing range of more difficult-to-access data, such as imaging, neuroscience, ecology, cohort data, systems biology and other new types of large-scale shareable data.
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