合成用于 pDNA 体内递送系统的 N-氧化物聚（1-乙烯基咪唑

IF 1.4 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Chemistry Letters Pub Date : 2024-04-16 DOI:10.1093/chemle/upae044

Ren Misaizu, Yoichi Negishi, Shoichiro Asayama

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引用次数: 0

摘要

摘要 N-氧化物聚（1-乙烯基咪唑）（PVIm+-O-）被合成为一种独特的生物相容性聚酰胺。溶液浊度的测量结果表明，PVIm+-O- 对血清蛋白快速聚合体的形成没有显著影响。琼脂糖凝胶延缓试验、粒度/ζ电位测量和细胞毒性评估证明，PVIm+-O-包覆了支链聚（乙烯亚胺）（bPEI）和质粒 DNA（pDNA）之间的二元复合物。在活体小鼠骨骼肌中，由此产生的 PVIm+-O-/bPEI/pDNA 三元复合物比 bPEI/pDNA 二元复合物能介导更高的基因表达。这些结果表明，PVIm+-O-是一种具有生物相容性的多聚体。

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Synthesis of N-oxide Poly(1-vinylimidazole) for pDNA delivery systems in vivo

Abstract N-oxide poly(1-vinylimidazole) (PVIm+–O−) has been synthesized as a unique biocompatible polyampholyte. Measurements of solution turbidity prove that PVIm+–O− had no significant effect on rapid aggregate formation with serum proteins. Agarose gel retardation assay, particle size/ζ-potential measurement, and cytotoxicity evaluation prove that the PVIm+–O− coated binary complexes between branched poly(ethyleneimine) (bPEI) and plasmid DNA (pDNA). The resulting PVIm+–O−/bPEI/pDNA ternary complex mediated higher gene expression than the bPEI/pDNA binary complex in vivo mouse skeletal muscle. These results suggest that PVIm+–O− is a polyampholyte for biocompatibility.

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来源期刊

Chemistry Letters 化学-化学综合

CiteScore

3.00

自引率

6.20%

发文量

260

审稿时长

1.2 months

期刊介绍： Chemistry Letters covers the following topics: -Organic Chemistry- Physical Chemistry- Inorganic Chemistry- Analytical Chemistry- Materials Chemistry- Polymer Chemistry- Supramolecular Chemistry- Organometallic Chemistry- Coordination Chemistry- Biomolecular Chemistry- Natural Products and Medicinal Chemistry- Electrochemistry