A. A. Kudryavtseva, A. V. Vlasov, E. V. Zinovev, D. D. Yanovskaya, A. A. Utkina, S. M. Rastorguev, I. V. Manukhov
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引用次数: 0
摘要
摘要ArdA DNA模拟抗限制蛋白通过代替DNA与RMI结合来抑制I型限制性修饰（RMI）系统。人们对 ArdA 与 RMI 复合物识别的 DNA 甲基化位点的特异性仍然知之甚少，即不清楚 ArdA 是否能模拟特定的 DNA 位点。从三种革兰氏阳性细菌中克隆了 ardA 基因：针对三种不同 DNA 识别/甲基化位点的大肠杆菌 RMI 系统测试了其产物的抗限制活性。虽然预测 ArdA 蛋白的结构相似，但它们对三种 RMI 系统都有很强的特异性。结果表明，DNA 拟态 ArdA 蛋白可模仿特定的 DNA 位点。
ArdA Protein Specificity against Type I Restriction–Modification Systems
Abstract
The ArdA DNA-mimic antirestriction proteins inhibit type I restriction−modification (RMI) systems by binding instead of DNA to RMI. The ArdA specificity to DNA methylation sites recognized by RMI complexes remains poorly understood; i.e., it is unclear whether a particular DNA site is mimicked by ArdA. The ardA genes were cloned from three Gram-positive bacteria: Agrobacterium tumefaciens, Pseudomonas monteilii, and Xanthomonas sp. Antirestriction activities of their products were tested against three Escherichia coli RMI systems differing in DNA recognition/methylation sites. Although similar structures were predicted for the ArdA proteins, the strong specificity to three RMI systems was observed. The results indicate that specific DNA sites may be imitated by DNA mimic ArdA proteins.
期刊介绍：
Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.
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