胸腺醌纳米粒子通过调节乳腺癌细胞系中的 SUMOylation 过程基因诱导细胞凋亡和延缓细胞迁移

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Behnoush Sohrabi, Mohaddese Qadbeigi, Farzaneh Sabouni, Ahmad Hamta
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引用次数: 0

摘要

背景:由于乳腺癌的异质性,大多数晚期患者对治疗产生抗药性。SUMO酰化(一种翻译后修饰)的破坏与乳腺癌有关:本研究旨在评估抗癌药物胸腺醌纳米粒子(Liposomal-TQ)与治疗乳腺癌最有效的化疗药物多柔比星(DXR)联合使用对正常和癌变乳腺细胞系中参与 SUMOylation 过程的两种主要成分 SENP2 和 SENP6 表达的影响:分别用 Liposomal-TQ 和 DXR 处理乳腺癌细胞 MCF7 和非肿瘤上皮细胞 MCF10。使用 MTT 和划痕试验评估细胞活力和细胞迁移。细胞凋亡分析采用 annexin-V/PI 染色法。使用实时定量 PCR(RT-qPCR)对 SENP2 和 SENP6 进行基因表达分析。此外,划痕试验评估了脂质体-TQ 的抗细胞迁移作用:RT-qPCR分析结果表明,与对照组相比,TQ和DXR处理组的SENP2和SENP6基因在MCF7细胞系中的表达量明显增加,但在MCF10细胞系中没有增加(P值<0.05)。此外,用脂质体-TQ处理MCF7和MCF10细胞24小时后,与对照组和脂质体组相比,晚期凋亡细胞显著增加(p值<0.0001),与对照组相比,DXR和脂质体-TQ均显著降低了乳腺癌细胞的迁移能力(p值分别为0.001和0.001):我们的研究表明,脂质体-TQ能促进乳腺癌细胞凋亡并抑制细胞迁移能力。这些发现加深了我们对脂质体-TQ 在乳腺癌 SUMOylation 通路中 SENP2 和 SENP6 的致癌活性中的作用的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Thymoquinone Nanoparticle Induces Apoptosis and Cell Migration Retardation through Modulating of SUMOylation Process Genes in Breast Cancer Cell Line.

Background: Due to the heterogeneity of breast cancer, most advanced-stage patients are resistant to therapy. Disruption of SUMOylation, a post-translational modification, is linked to breast cancer.

Objective: This study aimed to assess the impact of thymoquinone nanoparticles (Liposomal-TQ), an anti-cancer drug, combined with doxorubicin (DXR), the most effective chemotherapeutic drug used to treat breast cancer, on the expression of SENP2 and SENP6, two major components involved in the SUMOylation process, in normal and cancerous breast cell lines.

Materials and methods: The MCF7 cell line, a breast cancer cell line, and MCF10, a non-tumor epithelial cell line, were separately treated with Liposomal-TQ and DXR. Cell viability and cell migration were assessed using MTT and scratch tests. Apoptosis analysis was performed using annexin-V/PI staining. Gene expression analysis of SENP2 and SENP6 was conducted using quantitative real-time PCR (RT-qPCR). Additionally, the scratch test evaluated the anti-cell migratory effect of Liposomal-TQ.

Results: The findings obtained from RT-qPCR analysis indicated a significant increase in the expression of SENP2 and SENP6 genes in the TQ and DXR treatment groups compared to the control group in MCF7 but not in MCF10 cell lines (p-value < 0.05). Also, after 24 hours of treatment of MCF7 and MCF10 cells with liposomal-TQ, late apoptotic cells were significantly increased compared to the control and liposome groups (p-value < 0.0001) and compared to the control group, both DXR and Liposomal-TQ dramatically reduced the migratory ability of breast cancer cells (p-value = 0.001 and p-value = 0.001, respectively).

Conclusion: Our study indicated that Liposomal-TQ promotes apoptosis in breast cancer cells and inhibits cell migration ability. These findings enhance our understanding of the role of Liposomal-TQ in the carcinogenic activities of SENP2 and SENP6 in the SUMOylation pathway of breast cancer.

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来源期刊
Iranian Journal of Biotechnology
Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
2.60
自引率
7.70%
发文量
20
期刊介绍: Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.
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