{"title":"水稻 H2 型 RING E3 连接酶基因 OsSIRH2-3 通过维持 Na+/K+ 平衡积极调节耐盐性","authors":"Min Seok Choi, Ju Hee Kim, Cheol Seong Jang","doi":"10.1007/s12374-024-09433-9","DOIUrl":null,"url":null,"abstract":"<p>High soil salinity possesses a major challenge for plant growth and productivity. Plants have evolved various mechanisms to withstand the adverse effects of salt stress, including E3 ubiquitin ligases that label salt-responsive proteins for degradation. Here, we characterized the mechanisms RING E3 ubiquitin ligase OsSIRH2-3 (Oryza sativa Salt Induced RING H2-type-3 E3 ligase) used to facilitate salt tolerance in rice. <i>OsSIRH2-3</i> expression was upregulated under high NaCl concentrations and upon abscisic acid (ABA) treatment. OsSIRH2-3 was primarily found in the nucleus of rice protoplasts. The OsSIRH2-3 protein contains an H2-type-RING domain that confers E3 ligase activity. <i>OsSIRH2-3</i> overexpression was also found to be associated with enhanced salt tolerance in transgenic plants, decreased Na<sup>+</sup> accumulation in both roots and leaves, decreased Na<sup>+</sup> transport activity in the xylem sap, increased levels of proline and soluble sugars, elevated activity of reactive oxygen species scavenging enzymes, and altered expression of Na<sup>+</sup>/K<sup>+</sup> transporters. Furthermore, <i>OsSIRH2-3</i>-overexpressing plants also exhibited high sensitivity to exogenous ABA treatment. Our findings demonstrate that OsSIRH2-3 enhances salt tolerance by regulating Na<sup>+</sup>/K<sup>+</sup> homeostasis and modulating Na<sup>+</sup>/K<sup>+</sup> transporter expression. This study illuminates the molecular mechanisms involved in RING E3 ubiquitin ligase-mediated salt tolerance in rice and provides a potential strategy for enhancing crop productivity in saline environments.</p>","PeriodicalId":16762,"journal":{"name":"Journal of Plant Biology","volume":"74 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rice H2-Type RING E3 Ligase Gene, OsSIRH2-3, Positively Regulates Salt Tolerance by Maintaining Na+/K+ Homeostasis\",\"authors\":\"Min Seok Choi, Ju Hee Kim, Cheol Seong Jang\",\"doi\":\"10.1007/s12374-024-09433-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>High soil salinity possesses a major challenge for plant growth and productivity. Plants have evolved various mechanisms to withstand the adverse effects of salt stress, including E3 ubiquitin ligases that label salt-responsive proteins for degradation. Here, we characterized the mechanisms RING E3 ubiquitin ligase OsSIRH2-3 (Oryza sativa Salt Induced RING H2-type-3 E3 ligase) used to facilitate salt tolerance in rice. <i>OsSIRH2-3</i> expression was upregulated under high NaCl concentrations and upon abscisic acid (ABA) treatment. OsSIRH2-3 was primarily found in the nucleus of rice protoplasts. The OsSIRH2-3 protein contains an H2-type-RING domain that confers E3 ligase activity. <i>OsSIRH2-3</i> overexpression was also found to be associated with enhanced salt tolerance in transgenic plants, decreased Na<sup>+</sup> accumulation in both roots and leaves, decreased Na<sup>+</sup> transport activity in the xylem sap, increased levels of proline and soluble sugars, elevated activity of reactive oxygen species scavenging enzymes, and altered expression of Na<sup>+</sup>/K<sup>+</sup> transporters. Furthermore, <i>OsSIRH2-3</i>-overexpressing plants also exhibited high sensitivity to exogenous ABA treatment. Our findings demonstrate that OsSIRH2-3 enhances salt tolerance by regulating Na<sup>+</sup>/K<sup>+</sup> homeostasis and modulating Na<sup>+</sup>/K<sup>+</sup> transporter expression. This study illuminates the molecular mechanisms involved in RING E3 ubiquitin ligase-mediated salt tolerance in rice and provides a potential strategy for enhancing crop productivity in saline environments.</p>\",\"PeriodicalId\":16762,\"journal\":{\"name\":\"Journal of Plant Biology\",\"volume\":\"74 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12374-024-09433-9\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12374-024-09433-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
土壤高盐度是植物生长和生产力面临的一大挑战。植物已进化出多种机制来抵御盐胁迫的不利影响,其中包括标记盐反应蛋白以供降解的 E3 泛素连接酶。在这里,我们描述了 RING E3 泛素连接酶 OsSIRH2-3(Oryza sativa 盐诱导 RING H2- 型-3 E3 连接酶)用于促进水稻耐盐性的机制。在高浓度 NaCl 和脱落酸(ABA)处理条件下,OsSIRH2-3 的表达上调。OsSIRH2-3 主要存在于水稻原生质体的细胞核中。OsSIRH2-3 蛋白含有一个 H2 型-RING 结构域,具有 E3 连接酶活性。研究还发现,OsSIRH2-3 的过表达与转基因植株耐盐性增强、根和叶中 Na+ 积累减少、木质部汁液中 Na+ 运输活性降低、脯氨酸和可溶性糖含量增加、活性氧清除酶活性升高以及 Na+/K+ 转运体表达改变有关。此外,OsSIRH2-3 过表达植株对外源 ABA 处理也表现出高度敏感性。我们的研究结果表明,OsSIRH2-3可通过调节Na+/K+平衡和Na+/K+转运体的表达来增强耐盐性。这项研究揭示了 RING E3 泛素连接酶介导的水稻耐盐性的分子机制,为提高盐碱环境下作物的产量提供了一种潜在的策略。
Rice H2-Type RING E3 Ligase Gene, OsSIRH2-3, Positively Regulates Salt Tolerance by Maintaining Na+/K+ Homeostasis
High soil salinity possesses a major challenge for plant growth and productivity. Plants have evolved various mechanisms to withstand the adverse effects of salt stress, including E3 ubiquitin ligases that label salt-responsive proteins for degradation. Here, we characterized the mechanisms RING E3 ubiquitin ligase OsSIRH2-3 (Oryza sativa Salt Induced RING H2-type-3 E3 ligase) used to facilitate salt tolerance in rice. OsSIRH2-3 expression was upregulated under high NaCl concentrations and upon abscisic acid (ABA) treatment. OsSIRH2-3 was primarily found in the nucleus of rice protoplasts. The OsSIRH2-3 protein contains an H2-type-RING domain that confers E3 ligase activity. OsSIRH2-3 overexpression was also found to be associated with enhanced salt tolerance in transgenic plants, decreased Na+ accumulation in both roots and leaves, decreased Na+ transport activity in the xylem sap, increased levels of proline and soluble sugars, elevated activity of reactive oxygen species scavenging enzymes, and altered expression of Na+/K+ transporters. Furthermore, OsSIRH2-3-overexpressing plants also exhibited high sensitivity to exogenous ABA treatment. Our findings demonstrate that OsSIRH2-3 enhances salt tolerance by regulating Na+/K+ homeostasis and modulating Na+/K+ transporter expression. This study illuminates the molecular mechanisms involved in RING E3 ubiquitin ligase-mediated salt tolerance in rice and provides a potential strategy for enhancing crop productivity in saline environments.
期刊介绍:
Journal of Plant Biology, an official publication of the Botanical Society of Korea, is an international journal devoted to basic researches in biochemistry, cellular biology, development, ecology, genetics, molecular biology, physiology, and systematics of plants.
The Journal publishes the following categories of paper:
Original articles -- For publication in Journal of Plant Biology the manuscript must provide a significant new contribution to our understanding of plants. All areas of plant biology are welcome. No limit on the length, but a concise presentation is encouraged.
Reviews -- Invited by the EiC.
Brief Communications -- Concise but independent report representing significant contribution to plant science.
The Botanical Society of Korea was founded on November 30, 1957 to promote studies, disseminate and exchange information on the field of plant biology. The first issue of The Korean Journal of Botany, the official publication of the society, was published on April 1, 1958. It was published twice a year, but quarterly from 5th volume in 1962. In 1994, it was renamed to Journal of Plant Biology and published in English since 1996. The journal entered its 50th year of publication in 2007.