从丝瓜中提取的酶提取物:用于减少农业工业污染物和减轻毒性的前景广阔的生物催化剂

IF 2.8 3区 化学 Q2 CHEMISTRY, APPLIED
Clara Dourado Fernandes, Vera Lucia Scherholz Salgado de Castro, José Henrique Vallim, Atif Khurshid Wani, Juliana Heloisa Pinê Américo-Pinheiro, Teresa Serejo, Rinaldo Wellerson Pereira, Silvia Maria Egues, Luiz Fernando Romanholo Ferreira
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引用次数: 0

摘要

在生物降解的生物技术方法中,对土壤和水中的异种生物和难降解物质进行解毒的有效性引起了人们的极大兴趣。我们的研究采用了一种独特的方法,将白腐真菌(WRF)Pleurotus sajor-caju固定在丝瓜植物支架上。这种创新方法旨在促进对橘子产业产生的农用工业污染物浆洗的菌体修复。固定化过程大大提高了 MnP 酶活性,达到 23 IU.mL-1,Lac 活性约为 40.5 IU.mL-1。扫描电子显微镜和傅立叶变换红外光谱的定性分析深入揭示了微生物附着在载体上的机制,表明由于对木质纤维素结构的亲和力而发生了聚集,从而提高了多糖的产生,促进了生物催化剂的附着和潜在的可重复使用性。此外,从 P. sajor-caju 中提取富含木质素分解酶的酶提取物,显示了其降低纸浆洗涤毒性的能力。对柑橘废水毒性的研究表明,即使在低浓度下,也会产生严重畸形的胚胎,并抑制莴苣的发芽。值得注意的是,用酶提取物进行后处理后,对营养级的小白鼠和莴苣种子的毒性显著降低了 90%。这项研究极大地促进了对环境生物技术中真菌固定化策略的理解,强调了农业残留物作为酶生产的可持续诱导剂的潜力,以及它们在减轻农业工业废物对环境影响方面的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Enzymatic Extract from Luffa-Immobilized Pleurotus sajor-caju: A Promising Biocatalyst for Agro-Industrial Pollutant Reduction and Toxicity Mitigation

Enzymatic Extract from Luffa-Immobilized Pleurotus sajor-caju: A Promising Biocatalyst for Agro-Industrial Pollutant Reduction and Toxicity Mitigation

In biotechnological methods for biodegradation, the effectiveness of detoxifying xenobiotics and recalcitrant substances in soil and water has sparked significant interest. Our study takes a unique approach by focusing on immobilizing the white-rot fungus (WRF) Pleurotus sajor-caju onto a Luffa cylindrica plant support. This innovative method aims to facilitate the mycoremediation of agro-industrial pollutant pulp wash generated by the orange industry. The immobilization process significantly increased MnP enzymatic activity, reaching 23 IU.mL−1 and Lac activity at approximately 40.5 IU.mL−1. Qualitative SEM and FTIR analyses provided insights into the microorganism’s attachment mechanism to the support, suggesting that aggregation occurs due to an affinity to the lignocellulosic structure, enhancing the production of polysaccharides responsible for biocatalyst adherence and potential reusability. Furthermore, the production of an enzymatic extract rich in ligninolytic enzymes from P. sajor-caju showcased its ability to reduce the toxicity of pulp wash. An exploration into the toxicity of citrus effluent revealed the generation of embryos with severe deformities and the inhibition of Lactuca sativa germination, even at low concentrations. Notably, post-treatment with the enzymatic extract resulted in a remarkable 90% reduction in toxicity to the trophic level of Danio rerio and lettuce seeds. This research significantly contributes to understanding fungal immobilization strategies in environmental biotechnology, emphasizing the potential of agricultural residues as sustainable inducers for enzyme production and their pivotal role in mitigating the environmental impact of agro-industrial waste.

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来源期刊
Topics in Catalysis
Topics in Catalysis 化学-物理化学
CiteScore
5.70
自引率
5.60%
发文量
197
审稿时长
2 months
期刊介绍: Topics in Catalysis publishes topical collections in all fields of catalysis which are composed only of invited articles from leading authors. The journal documents today’s emerging and critical trends in all branches of catalysis. Each themed issue is organized by renowned Guest Editors in collaboration with the Editors-in-Chief. Proposals for new topics are welcome and should be submitted directly to the Editors-in-Chief. The publication of individual uninvited original research articles can be sent to our sister journal Catalysis Letters. This journal aims for rapid publication of high-impact original research articles in all fields of both applied and theoretical catalysis, including heterogeneous, homogeneous and biocatalysis.
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