甘蓝(Brassica oleracea ssp. capitata L.)在春化过程中的时程转录组分析

IF 2.2 4区 生物学 Q2 PLANT SCIENCES
Heewon Moon, Byoung-Ho Hwang, Minkyu Park, Enamul Huq, Dong-Hwan Kim
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引用次数: 0

摘要

许多二年生和多年生植物在冬季长期暴露于寒冷环境中,即所谓的春化现象,会引发其从无性生殖阶段向生殖阶段的过渡。在过去几十年中,深入的研究揭示了这一现象的分子机制,特别是利用拟南芥模式植物。包括拟南芥在内的大多数十字花科植物都需要春化来实现花期转换。十字花科植物可分为两类:种子春化反应型和植物春化反应型。卷心菜属于植物春化反应型。关于卷心菜对春化的植物春化反应性状的分子细节仍不甚明了。本研究对甘蓝近交系'BN2348'春化反应的转录组进行了分析。与拟南芥的情况类似,甘蓝中的两个 VIN3 同源物(BoVIN3.C3 和 BoVIN3.C2)在暴露于长期低温时被高度诱导。我们的转录组分析发现,两个FT同源物(BoFT.C2和BoFT.C6)和三个SOC1同源物(BoSOC1.1.C4、BoSOC1.2.C4和BoSOC1.C3)具有调控油菜花转化的功能。此外,通过系统发育和同源分析,在油菜基因组中发现了 5 个 FLC 同源物,分别命名为 BoFLC1.a、BoFLC1.b、BoFLC2、BoFLC3 和 BoFLC5。转录组分析表明,这些基因可分为春化响应基因(BoFLC2 和 BoFLC3)和春化不敏感基因(BoFLC1.a、BoFLC1.b 和 BoFLC5)。作为春化类型的绿色植物,幼苗中存在春化不敏感的 BoFLC 同源物可能是大白菜比大白菜和拟南芥等种子春化类型植物暴露于低温时间更长的原因。这些发现加深了我们对卷心菜植物花期转换的分子动力学基础的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Time Course Transcriptomic Analysis of Cabbage (Brassica oleracea ssp. capitata L.) During Vernalization

Time Course Transcriptomic Analysis of Cabbage (Brassica oleracea ssp. capitata L.) During Vernalization

Long-term exposure to cold during the winter season, so-called vernalization, triggers the transition from the vegetative to the reproductive stage in many biennial and perennial plants. In the last decades, intensive researches have revealed the molecular mechanisms underlying this phenomenon, particularly using Arabidopsis model plant. Most Brassicaceae family plants, including the Arabidopsis, require vernalization for floral transition. Brassicaceae family plants can be classified into two groups: seed vernalization responsive type and plant vernalization responsive type. Cabbage belongs to plant vernalization responsive type. Molecular details on plant vernalization responsive trait of cabbage on vernalization are still poorly understood. In this study, we conducted a transcriptomic analysis of the cabbage inbred line ‘BN2348’ in response to vernalization. Similar to the case of Arabidopsis, two VIN3 homologs (BoVIN3.C3 and BoVIN3.C2) were highly induced by the exposure to long-term cold in B. oleracea. Our transcriptome analysis identified that two FT homologs (BoFT.C2 and BoFT.C6) and three SOC1 homologs (BoSOC1.1.C4, BoSOC1.2.C4, and BoSOC1.C3) were functioning for the regulation of floral transition in B. oleracea. In addition, by phylogenic and syntenic analyses, a total of five FLC homologs, named BoFLC1.a, BoFLC1.b, BoFLC2, BoFLC3, and BoFLC5, were identified in the genome of B. oleracea. Transcriptomic analysis indicated that these genes could be grouped into vernalization-responsive (BoFLC2 and BoFLC3) and vernalization-insensitive genes (BoFLC1.a, BoFLC1.b, and BoFLC5). As green plant vernalization type, it might suggest the existence of vernalization-insensitive BoFLC homologs in young seedlings might be the reason why cabbage exhibits longer exposure of cold compared to seed vernalization type plants such as Chinese cabbage and Arabidopsis. These findings improve our understanding of the molecular dynamics underlying floral transition in cabbage plants.

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来源期刊
Journal of Plant Biology
Journal of Plant Biology 生物-植物科学
CiteScore
5.40
自引率
3.40%
发文量
29
审稿时长
1 months
期刊介绍: Journal of Plant Biology, an official publication of the Botanical Society of Korea, is an international journal devoted to basic researches in biochemistry, cellular biology, development, ecology, genetics, molecular biology, physiology, and systematics of plants. The Journal publishes the following categories of paper: Original articles -- For publication in Journal of Plant Biology the manuscript must provide a significant new contribution to our understanding of plants. All areas of plant biology are welcome. No limit on the length, but a concise presentation is encouraged. Reviews -- Invited by the EiC. Brief Communications -- Concise but independent report representing significant contribution to plant science. The Botanical Society of Korea was founded on November 30, 1957 to promote studies, disseminate and exchange information on the field of plant biology. The first issue of The Korean Journal of Botany, the official publication of the society, was published on April 1, 1958. It was published twice a year, but quarterly from 5th volume in 1962. In 1994, it was renamed to Journal of Plant Biology and published in English since 1996. The journal entered its 50th year of publication in 2007.
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