三倍体和益生菌疗法(双歧杆菌、乳酸杆菌和乳球菌混合物)对幼年鲑鱼(Oncorhynchus tshawytscha)行为转录组学的影响。

IF 2.4 4区 心理学 Q2 BEHAVIORAL SCIENCES
Chelsea E. Frank, Javad Sadeghi, Daniel D. Heath, Christina A. D. Semeniuk
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引用次数: 0

摘要

水产养殖者使用多倍体鱼来最大限度地提高产量,尽管会带来一些意想不到的后果,包括行为和生理功能受损。鉴于益生菌疗法的益处(如改善免疫反应、生长和新陈代谢),我们探索了益生菌补充剂(双歧杆菌、乳酸杆菌和乳球菌的混合物),以克服其缺点。首先,我们使用 16S 代谢编码(通过主坐标分析和 PERMANOVA)研究了鱼类肠道细菌群落组成,并确定益生菌对肠道细菌组成有显著影响(p = 0.001)。其次,我们研究了基因组干扰物(三倍体)和饮食补充剂(益生菌)如何影响孵化场饲养的大鳞鲑(Oncorhynchus tshawytscha)的基因转录和行为特征。来自四个处理组(二倍体-常规饲料组、二倍体-益生菌饲料组、三倍体-常规饲料组和三倍体-益生菌饲料组;n = 360)的幼鱼接受了行为测定,以测试活动性、探索性、恐新性、捕食者规避性、攻击性/社会性、行为敏感性和灵活性。在这些鱼类中,(i) 通过主成分分析和一般线性混合模型检查了与神经功能(神经发生/突触可塑性)和应激反应及发育(生长/食欲)生物标志物相关的基因转录谱,(ii) 用于描述行为表型。三倍体表现出更活跃的行为特征(p = 0.002),正常饮食的三倍体有更多的神经肽 Y 转录(p = 0.02)。生长基因(早期生长反应蛋白 1,p = 0.02)和长期神经发育基因(神经源分化因子,p = 0.003 和突触体相关蛋白 25-a,p = 0.005)分别对活动和反应特征产生影响。总体而言,我们的益生菌处理并不能补偿三倍体。我们的研究突显了行为转录组学在确定候选基因以及与复杂行为曲目的动态、机制关联方面的新应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Behavioral transcriptomic effects of triploidy and probiotic therapy (Bifidobacterium, Lactobacillus, and Lactococcus mixture) on juvenile Chinook salmon (Oncorhynchus tshawytscha)

Behavioral transcriptomic effects of triploidy and probiotic therapy (Bifidobacterium, Lactobacillus, and Lactococcus mixture) on juvenile Chinook salmon (Oncorhynchus tshawytscha)

Aquaculturists use polyploid fish to maximize production albeit with some unintended consequences including compromised behaviors and physiological function. Given benefits of probiotic therapies (e.g., improved immune response, growth, and metabolism), we explored probiotic supplementation (mixture of Bifidobacterium, Lactobacillus, and Lactococcus), to overcome drawbacks. We first examined fish gut bacterial community composition using 16S metabarcoding (via principal coordinate analyses and PERMANOVA) and determined probiotics significantly impacted gut bacteria composition (p = 0.001). Secondly, we examined how a genomic disruptor (triploidy) and diet supplements (probiotics) impact gene transcription and behavioral profiles of hatchery-reared Chinook salmon (Oncorhynchus tshawytscha). Juveniles from four treatment groups (diploid-regular feed, diploid-probiotic feed, triploid-regular feed, and triploid-probiotic feed; n = 360) underwent behavioral assays to test activity, exploration, neophobia, predator evasion, aggression/sociality, behavioral sensitivity, and flexibility. In these fish, transcriptional profiles for genes associated with neural functions (neurogenesis/synaptic plasticity) and biomarkers for stress response and development (growth/appetite) were (i) examined across treatments and (ii) used to describe behavioral phenotypes via principal component analyses and general linear mixed models. Triploids exhibited a more active behavioral profile (p = 0.002), and those on a regular diet had greater Neuropeptide Y transcription (p = 0.02). A growth gene (early growth response protein 1, p = 0.02) and long-term neural development genes (neurogenic differentiation factor, p = 0.003 and synaptysomal-associated protein 25-a, p = 0.005) impacted activity and reactionary profiles, respectively. Overall, our probiotic treatment did not compensate for triploidy. Our research highlights novel applications of behavioral transcriptomics for identifying candidate genes and dynamic, mechanistic associations with complex behavioral repertoires.

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来源期刊
Genes Brain and Behavior
Genes Brain and Behavior 医学-行为科学
CiteScore
6.80
自引率
4.00%
发文量
62
审稿时长
4-8 weeks
期刊介绍: Genes, Brain and Behavior was launched in 2002 with the aim of publishing top quality research in behavioral and neural genetics in their broadest sense. The emphasis is on the analysis of the behavioral and neural phenotypes under consideration, the unifying theme being the genetic approach as a tool to increase our understanding of these phenotypes. Genes Brain and Behavior is pleased to offer the following features: 8 issues per year online submissions with first editorial decisions within 3-4 weeks and fast publication at Wiley-Blackwells High visibility through its coverage by PubMed/Medline, Current Contents and other major abstracting and indexing services Inclusion in the Wiley-Blackwell consortial license, extending readership to thousands of international libraries and institutions A large and varied editorial board comprising of international specialists.
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