{"title":"增强从地衣芽孢杆菌中提取的脂肽作为益生菌对 MDR 鲍曼不动杆菌的抗菌作用","authors":"Mahdi Hosseini Bafghi, Farangis Ghanipour, Razieh Nazari, Seyed Soheil Aghaei, Parvaneh Jafari","doi":"10.31083/j.fbl2905171","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The antibiotic resistance of microorganisms is escalating rapidly. Infections caused by opportunistic pathogens in immunocompromised individuals have prompted researchers to seek for potent and safe antibacterial agents. The purpose of this investigation was to explore the suppression of virulence gene expression, specifically the <i>pga</i> operon genes responsible in biofilm formation in <i>Acinetobacter baumannii</i>, through the utilization of metabolites obtained from probiotic bacteria.</p><p><strong>Methods: </strong>To assess the antimicrobial properties, standard strains of five probiotic bacteria were tested against a standard strain of multidrug-resistant (MDR) <i>A. baumannii</i> employing the agar gel diffusion technique. Following the identification of the most potent probiotic strain (<i>Bacillus licheniformis</i>), the existence of its <i>LanA</i> and <i>LanM</i> genes was confirmed using the polymerase chain reaction (PCR) test. High-performance liquid chromatography (HPLC) and fourier-transform infrared spectroscopy (FTIR) techniques were employed to identify the intended metabolite, which was found to be a lipopeptide nature. The minimum inhibitory concentration (MIC) values and anti-biofilm activity of the targeted metabolite were determined using a dilution method in 96-well microplates and field emission scanning electron microscopy (FE-SEM). Real-time PCR (qPCR) was utilized for comparing the expression of <i>pga</i> operon genes, including <i>pgaABCD</i>, in <i>A. baumannii</i> pre- and post-exposure to the derived lipopeptide.</p><p><strong>Results: </strong>The MIC results indicated that the probiotic product inhibited the growth of <i>A. baumannii</i> at concentrations lower than those needed for conventional antibiotics. Furthermore, it was observed that the desired genes' expression decreased due to the effect of this substance.</p><p><strong>Conclusions: </strong>This research concludes that the <i>B. licheniformis</i> probiotic product could be a viable alternative for combating drug resistance in <i>A. baumannii</i>.</p>","PeriodicalId":73069,"journal":{"name":"Frontiers in bioscience (Landmark edition)","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhancing the Antibacterial Impact of Lipopeptide Extracted from <i>Bacillus licheniformis</i> as a Probiotic against MDR <i>Acinetobacter baumannii</i>.\",\"authors\":\"Mahdi Hosseini Bafghi, Farangis Ghanipour, Razieh Nazari, Seyed Soheil Aghaei, Parvaneh Jafari\",\"doi\":\"10.31083/j.fbl2905171\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The antibiotic resistance of microorganisms is escalating rapidly. Infections caused by opportunistic pathogens in immunocompromised individuals have prompted researchers to seek for potent and safe antibacterial agents. The purpose of this investigation was to explore the suppression of virulence gene expression, specifically the <i>pga</i> operon genes responsible in biofilm formation in <i>Acinetobacter baumannii</i>, through the utilization of metabolites obtained from probiotic bacteria.</p><p><strong>Methods: </strong>To assess the antimicrobial properties, standard strains of five probiotic bacteria were tested against a standard strain of multidrug-resistant (MDR) <i>A. baumannii</i> employing the agar gel diffusion technique. Following the identification of the most potent probiotic strain (<i>Bacillus licheniformis</i>), the existence of its <i>LanA</i> and <i>LanM</i> genes was confirmed using the polymerase chain reaction (PCR) test. High-performance liquid chromatography (HPLC) and fourier-transform infrared spectroscopy (FTIR) techniques were employed to identify the intended metabolite, which was found to be a lipopeptide nature. The minimum inhibitory concentration (MIC) values and anti-biofilm activity of the targeted metabolite were determined using a dilution method in 96-well microplates and field emission scanning electron microscopy (FE-SEM). Real-time PCR (qPCR) was utilized for comparing the expression of <i>pga</i> operon genes, including <i>pgaABCD</i>, in <i>A. baumannii</i> pre- and post-exposure to the derived lipopeptide.</p><p><strong>Results: </strong>The MIC results indicated that the probiotic product inhibited the growth of <i>A. baumannii</i> at concentrations lower than those needed for conventional antibiotics. Furthermore, it was observed that the desired genes' expression decreased due to the effect of this substance.</p><p><strong>Conclusions: </strong>This research concludes that the <i>B. licheniformis</i> probiotic product could be a viable alternative for combating drug resistance in <i>A. baumannii</i>.</p>\",\"PeriodicalId\":73069,\"journal\":{\"name\":\"Frontiers in bioscience (Landmark edition)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in bioscience (Landmark edition)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31083/j.fbl2905171\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in bioscience (Landmark edition)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31083/j.fbl2905171","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Enhancing the Antibacterial Impact of Lipopeptide Extracted from Bacillus licheniformis as a Probiotic against MDR Acinetobacter baumannii.
Background: The antibiotic resistance of microorganisms is escalating rapidly. Infections caused by opportunistic pathogens in immunocompromised individuals have prompted researchers to seek for potent and safe antibacterial agents. The purpose of this investigation was to explore the suppression of virulence gene expression, specifically the pga operon genes responsible in biofilm formation in Acinetobacter baumannii, through the utilization of metabolites obtained from probiotic bacteria.
Methods: To assess the antimicrobial properties, standard strains of five probiotic bacteria were tested against a standard strain of multidrug-resistant (MDR) A. baumannii employing the agar gel diffusion technique. Following the identification of the most potent probiotic strain (Bacillus licheniformis), the existence of its LanA and LanM genes was confirmed using the polymerase chain reaction (PCR) test. High-performance liquid chromatography (HPLC) and fourier-transform infrared spectroscopy (FTIR) techniques were employed to identify the intended metabolite, which was found to be a lipopeptide nature. The minimum inhibitory concentration (MIC) values and anti-biofilm activity of the targeted metabolite were determined using a dilution method in 96-well microplates and field emission scanning electron microscopy (FE-SEM). Real-time PCR (qPCR) was utilized for comparing the expression of pga operon genes, including pgaABCD, in A. baumannii pre- and post-exposure to the derived lipopeptide.
Results: The MIC results indicated that the probiotic product inhibited the growth of A. baumannii at concentrations lower than those needed for conventional antibiotics. Furthermore, it was observed that the desired genes' expression decreased due to the effect of this substance.
Conclusions: This research concludes that the B. licheniformis probiotic product could be a viable alternative for combating drug resistance in A. baumannii.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
