蠵龟是多种细菌和真菌群落的宿主。

IF 3.3 3区 生物学 Q2 ECOLOGY
Klara Filek, Borna Branimir Vuković, Marta Žižek, Lucija Kanjer, Adriana Trotta, Antonio Di Bello, Marialaura Corrente, Sunčica Bosak
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引用次数: 0

摘要

与野生动物相关的微生物群落研究提供了一个宝贵的知识库,可用于加强野生动物的康复和保护。蠵龟(Caretta caretta)是一种分布于全球的物种,其地中海种群被世界自然保护联盟(IUCN)濒危物种红色名录列为最不受关注物种。在我们的研究中,我们的目的是进一步了解其生物学与其相关微生物的关系。我们调查了泄殖腔、口腔粘膜、甲壳生物膜的表生和内生细菌及内生真菌群落。通过对 16S rRNA 基因和核糖体基因 ITS2 区域进行扩增片段测序,分析了从 18 只幼龟、亚成龟和成龟以及 8 个相应的围栏中获取的样本,这些样本的时间跨度为 3 年。结果表明,随着海龟年龄的增长,远端肠道细菌群落的多样性呈下降趋势。值得注意的是,Tenacibaculum物种在幼龟中的相对丰度高于成龟。在泄殖腔和口腔样本中都观察到了不同丰度的Tenacibaculum、Moraxellaceae、Cardiobacteriaceae和Campylobacter类群,此外还有不同的微生物组成,Halioglobus类群只出现在口腔样本中。蠵龟泄殖腔中的真菌群落种类繁多,个体之间差异显著,与水箱水中的真菌群落不同。我们的研究结果扩大了蠵龟微生物多样性的已知范围,突出了个体身体部位特有的有趣类群。这项研究提供了蠵龟细菌微生物群的一个全面视角,并首次报道了远端肠道真菌群落,有助于建立对蠵龟整体菌群的基本认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Loggerhead Sea Turtles as Hosts of Diverse Bacterial and Fungal Communities.

Loggerhead Sea Turtles as Hosts of Diverse Bacterial and Fungal Communities.

Research on microbial communities associated with wild animals provides a valuable reservoir of knowledge that could be used for enhancing their rehabilitation and conservation. The loggerhead sea turtle (Caretta caretta) is a globally distributed species with its Mediterranean population categorized as least concern according to the IUCN Red List of Threatened Species as a result of robust conservation efforts. In our study, we aimed to further understand their biology in relation to their associated microorganisms. We investigated epi- and endozoic bacterial and endozoic fungal communities of cloaca, oral mucosa, carapace biofilm. Samples obtained from 18 juvenile, subadult, and adult turtles as well as 8 respective enclosures, over a 3-year period, were analysed by amplicon sequencing of 16S rRNA gene and ITS2 region of nuclear ribosomal gene. Our results reveal a trend of decreasing diversity of distal gut bacterial communities with the age of turtles. Notably, Tenacibaculum species show higher relative abundance in juveniles than in adults. Differential abundances of taxa identified as Tenacibaculum, Moraxellaceae, Cardiobacteriaceae, and Campylobacter were observed in both cloacal and oral samples in addition to having distinct microbial compositions with Halioglobus taxa present only in oral samples. Fungal communities in loggerheads' cloaca were diverse and varied significantly among individuals, differing from those of tank water. Our findings expand the known microbial diversity repertoire of loggerhead turtles, highlighting interesting taxa specific to individual body sites. This study provides a comprehensive view of the loggerhead sea turtle bacterial microbiota and marks the first report of distal gut fungal communities that contributes to establishing a baseline understanding of loggerhead sea turtle holobiont.

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来源期刊
Microbial Ecology
Microbial Ecology 生物-海洋与淡水生物学
CiteScore
6.90
自引率
2.80%
发文量
212
审稿时长
3-8 weeks
期刊介绍: The journal Microbial Ecology was founded more than 50 years ago by Dr. Ralph Mitchell, Gordon McKay Professor of Applied Biology at Harvard University in Cambridge, MA. The journal has evolved to become a premier location for the presentation of manuscripts that represent advances in the field of microbial ecology. The journal has become a dedicated international forum for the presentation of high-quality scientific investigations of how microorganisms interact with their environment, with each other and with their hosts. Microbial Ecology offers articles of original research in full paper and note formats, as well as brief reviews and topical position papers.
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