细胞迁移实验中封闭微通道的制作与应用综述

IF 1.9 4区 工程技术 Q2 Engineering
Mazlee Bin Mazalan, Ryota Toyohara, Toshiro Ohashi
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引用次数: 0

摘要

细胞迁移是许多生理和病理事件中的一个重要过程,而且已知会受到外部微环境的调控,因为细胞会感知来自微环境的物理和化学信号,并对这些信号做出集体反应。过去二十年来,人们一直致力于研究外部微环境如何影响细胞迁移行为。在体内,细胞经常在受限环境中迁移,如组织中的细胞外基质和毛细血管。了解细胞如何在这些受限空间中移动对阐明各种生物过程至关重要。例如,在胚胎发育过程中,细胞通过特定途径迁移以形成组织和器官。在伤口愈合过程中,细胞迁移以修复受损组织。在癌症中,肿瘤细胞迁移到周围组织并转移到远处。生物微机电系统(Bio-MEMS)技术的最新进展使人们能够在微尺度上描述细胞力学特性并控制局部细胞环境。为了研究封闭条件下的细胞迁移,微通道因其方向性和兼容性而被广泛制造和使用。因此,本研究综述了近期有关微通道制造及其在研究细胞迁移行为中的应用的研究成果,包括直通道和迂回结构。本研究还讨论了与研究微通道中细胞迁移相关的挑战和局限性。研究细胞在封闭环境中的迁移可为了解细胞迁移的基本机制提供有价值的见解,并有助于制定治疗干预策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A Review of Fabrication and Applications of Confined Microchannels for Cell Migration Assay

A Review of Fabrication and Applications of Confined Microchannels for Cell Migration Assay

Cell migration is an essential process in a number of physiological and pathological events, and known to be modulated by external microenvironment because cells may sense physical and chemical signals from the microenvironment and collectively respond to these signals. Over the past two decades, a lot of efforts have been made to study how external microenvironment can affect cell migration behaviors. Cells often migrate through confined environments in vivo, such as extracellular matrices in tissues and capillary vessels. Understanding how cells move in these constrained spaces is crucial to clarify various biological processes. For instance, during embryonic development, cells migrate through specific pathways to form tissues and organs. In wound healing, cells migrate to repair damaged tissues. In cancer, tumour cells migrate to invade surrounding tissues and metastasize to distant sites. Recent advances of bio-MEMS technologies have enabled to characterize cell mechanics and to control local cellular environment at micro-scale. In order to study cell migration under confinement, microchannels have been widely fabricated and used due to their directionality and compatibility. Thus, this study reviews recent work on fabrication of microchannels and their applications to investigate cell migration behaviors, ranging from straight channels to tortuous structures. Challenges and limitations associated with studying cell migration in microchannels are also discussed. Reviewing cell migration in confined environments may provide valuable insights into the underlying mechanisms of cell migration and aid in developing strategies for therapeutic interventions.

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来源期刊
CiteScore
4.10
自引率
10.50%
发文量
115
审稿时长
3-6 weeks
期刊介绍: The International Journal of Precision Engineering and Manufacturing accepts original contributions on all aspects of precision engineering and manufacturing. The journal specific focus areas include, but are not limited to: - Precision Machining Processes - Manufacturing Systems - Robotics and Automation - Machine Tools - Design and Materials - Biomechanical Engineering - Nano/Micro Technology - Rapid Prototyping and Manufacturing - Measurements and Control Surveys and reviews will also be planned in consultation with the Editorial Board.
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