通过 PEG 化增强绿色合成硒纳米粒子的抗乳腺癌活性:诱导细胞凋亡和潜在的抗癌药物输送系统

IF 1.7 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY
Samer Y Al-Qaraleh, Wael A Al-Zereini, Sawsan A Oran, Osama Y Al-Madanat, Aiman I Al-Qtaitat and Abdalrahim Alahmad
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引用次数: 0

摘要

乳腺癌是全世界发病率和死亡率都很高的一种疾病。生物纳米粒子(尤其是硒纳米粒子(SeNPs))作为替代抗癌剂的潜在用途已在多项研究中得到广泛认可。研究人员使用辣木的水提取物合成了纳米颗粒(MPM-SeNPs),并对其进行了 PEG 化处理(PEG-MPM-SeNPs)。MPM-SeNPs 采用化学和物理技术进行表征。通过分光光度测量和傅立叶变换红外光谱(FT-IR)分析,证实了 MPM-SeNPs 与 PEG 的成功封装。此外,还评估了 PEG 化 MPM-SeNPs 对提高其抗乳腺癌活性和作为药物递送剂的效果。因此,测量了 DOX 在不同 pH 值下的负载效率和释放量;评估了 PEG-MPM-SeNPs 对腺癌乳腺癌细胞系(MDA-MB-231)的抗增殖活性,并与生物源 MPM-SeNPs 和 DOX 共轭 PEG-MPM-SeNPs 的抗增殖活性进行了比较。与 MPM-SeNPs 相比,PEG-MPM-SeNPs 和 DOX-PEG-MPM-SeNPs 的 IC50 值有所降低;IC50 分别为 11.54 ± 1.74 和 31.27 ± 2.9 μg mL-1 ,而 MPM-SeNPs 为 71.4 ± 3.4 μg mL-1。MPM-SeNPs 和 PEG-MPM-SeNPs 可导致 MDA-MB-231 细胞凋亡,线粒体膜电位(MMP)显著降低,释放的细胞色素 C(Cyt C)增加,Caspase-3/9 被激活(P < 0.05)。将 DOX 与 PEG-MPM-SeNPs 连接会导致 Caspase-3/8 浓度增加和释放的细胞色素 C 增加,但在处理过的和未处理过的对照组癌细胞之间,MMP 的差异并不显著(P > 0.1)。MPM-SeNPs和PEG-MPM-SeNPs通过内在途径导致凋亡反应,而将DOX与PEG-MPM-SeNPs连接则通过外在途径导致癌细胞凋亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhanced anti-breast cancer activity of green synthesized selenium nanoparticles by PEGylation: induction of apoptosis and potential anticancer drug delivery system
Breast cancer is a disease associated with high morbidity and mortality rates worldwide. The potential use of biogenic nanoparticles as alternative anticancer agents has been immensely acknowledged in several studies, particularly selenium nanoparticles (SeNPs). Nanoparticles were synthesised using the aqueous extract of Moringa peregrine (MPM-SeNPs) and were PEGylated (PEG-MPM-SeNPs). MPM-SeNPs were characterised by chemical and physical techniques. The successful capping of MPM-SeNPs with PEG was confirmed by spectrophotometric measurements and via Fourier-transform infrared spectroscopy (FT-IR) analysis. Furthermore, the effect of PEGylation of MPM-SeNPs on enhancing their anti-breast cancer activity and as a drug delivery agent was evaluated. Therefore, the loading efficiency and release of DOX at different pH values were measured; the antiproliferative activity of PEG-MPM-SeNPs against the adenocarcinoma breast cancer cell line (MDA-MB-231) was evaluated and compared with that of biogenic MPM-SeNPs and DOX-conjugated PEG-MPM-SeNPs. PEG-MPM-SeNPs and DOX-PEG-MPM-SeNPs had reduced IC50 values compared to MPM-SeNPs; IC50 of 11.54 ± 1.74 and 31.27 ± 2.9 μg mL−1 compared to 71.4 ± 3.4 μg mL−1, respectively. MPM-SeNPs and PEG-MPM-SeNPs caused apoptosis to MDA-MB-231 cells with a significant decrease in the mitochondrial membrane potential (MMP), increase in the released cytochrome C (Cyt C), and activation of caspase-3/9 (P < 0.05). Linking DOX to PEG-MPM-SeNPs led to an increase in caspase-3/8 concentrations and an increase in the released Cyt C, but there were non-significant differences in MMP (P > 0.1) between treated and untreated control cancer cells. MPM-SeNPs and PEG-MPM-SeNPs caused apoptotic reactions via an intrinsic pathway, while linking DOX to PEG-MPM-SeNPs caused apoptosis in cancer cells through an extrinsic pathway.
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来源期刊
Advances in Natural Sciences: Nanoscience and Nanotechnology
Advances in Natural Sciences: Nanoscience and Nanotechnology NANOSCIENCE & NANOTECHNOLOGYMATERIALS SCIE-MATERIALS SCIENCE, MULTIDISCIPLINARY
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