5-HT1A 受体拮抗剂和 5-HT2A 受体激动剂对吗啡戒断的影响

IF 0.5 4区 医学 Q4 NEUROSCIENCES
Mahdi Ramezani, Siamak Shahidi, Simin Afshar, Parisa Habibi, Nasrin Hashemi-Firouzi
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引用次数: 0

摘要

摘要 长期服用吗啡会导致身体依赖,这可能是通过5-羟色胺5-HT1A受体(5-HT1AR)和5-HT2A受体(5-HT1AR)引起的。本研究旨在评估 5-HT1AR 拮抗剂盐酸 NAD 299(NAD 299)和 5-HT2A R 激动剂 TCB-2 对吗啡依赖小鼠戒断综合征的影响。成年雄性小鼠被随机分为五组。三组小鼠在注射纳洛酮(3 毫克/千克,静脉注射)前 15 分钟分别接受 NAD-299 (0.3 毫克/千克,静脉注射)和 TCB-2(0.3 毫克/千克,静脉注射)治疗,以研究 5-HT1AR 拮抗剂和 5-HT2AR 激动剂对吗啡戒断综合征的影响。吗啡皮下注射,每日剂量递增,间隔12小时,连续5天,以诱导吗啡依赖。在30分钟内记录戒断症状,包括跳跃、腹部蠕动、体重减轻和摇头。吗啡戒断试验2小时后评估皮质醇和总抗氧化剂水平。与吗啡组相比,NAD-299 + 吗啡组的戒断总分降低,摇头和总抗氧化能力增加。与吗啡组相比,TCB-2 + 吗啡组的跳跃增加,扭动、摇头和戒断评分减少。与吗啡组相比,NAD-299+TCB-2+吗啡组的跳跃次数和戒断总分减少,而摇头和皮质醇水平增加。NAD-299和TCB-2的联合治疗可调节吗啡戒断综合征并提高皮质醇水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of 5-HT1A Receptor Antagonist and 5-HT2A Receptor Agonist on Morphine Withdrawal

Effects of 5-HT1A Receptor Antagonist and 5-HT2A Receptor Agonist on Morphine Withdrawal

Abstract

Chronic administration of morphine causes physical dependence, possibly through serotonin 5‑HT1A receptor (5-HT1AR) and 5-HT2A receptor (5-HT1AR). The aim of this study was to evaluate the effect of 5-HT1AR antagonist, NAD 299 hydrochloride (NAD 299), and 5-HT2A R agonist (TCB-2) on the withdrawal syndrome in morphine-dependent mice. Adult male mice were randomly divided into five groups. Three groups of mice were treated with NAD-299 (0.3 mg/kg, i.p.) and TCB-2 (0.3 mg/kg, i.p.) 15 min before naloxone injection (3 mg/kg, s.c.) to investigate the effect of the 5-HT1AR antagonist and 5-HT2AR agonist on morphine withdrawal syndrome. Morphine was administered subcutaneously with increasing daily doses at 12-h intervals for five days to induce dependence. The withdrawal symptoms, including jumping, abdomen writhing, body weight loss, and head shakes, were recorded for 30 min. Cortisol and total antioxidant levels were assessed 2 h after the morphine withdrawal test. There was a reduction in total withdrawal score and an increase in head shakes and total antioxidant capacity in the NAD-299 + morphine group compared to the morphine group. The TCB-2 + morphine group exhibited an increase in jumping and a decrease in writhing, head shakes, and withdrawal score compared to the morphine group. The NAD-299 + TCB-2 + morphine group showed a decrease in the number of jumping and total withdrawal score, and an increase in the head shakes and cortisol levels compared to the morphine group. The combined treatment with NAD-299 and TCB-2 modulates the morphine withdrawal syndrome and increases cortisol levels.

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来源期刊
Neurochemical Journal
Neurochemical Journal 医学-神经科学
自引率
20.00%
发文量
40
审稿时长
>12 weeks
期刊介绍: Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components. The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.
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