牙髓干细胞调节培养基对抗 RANKL 抗体相关性颌骨坏死的预防作用

IF 3.3 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Calcified Tissue International Pub Date : 2024-08-01 Epub Date: 2024-05-29 DOI:10.1007/s00223-024-01232-1
Kento Kaminogo, Satoshi Yamaguchi, Hui Chen, Hideo Yagita, Naoto Toyama, Yusuke Urata, Hideharu Hibi
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引用次数: 0

摘要

与药物相关的颌骨坏死是一种严重的疾病,多发于正在接受抗骨质吸收剂(ARA)治疗的癌症和骨质疏松症患者,如双磷酸盐(BP)或针对NF-κB配体受体激活剂的抗体--地诺单抗。最近,以干细胞为基础的疗法已被证明可有效预防与双膦酸盐相关的颌骨坏死的发生。然而,对地诺单抗相关颌骨坏死(DRONJ)的研究仍然有限。本文评估了牙髓干细胞条件培养基(DPSC-CM)在小鼠模型中预防DRONJ的疗效。在患有DRONJ的小鼠拔牙窝局部施用DPSC-CM可减少空骨细胞空隙的数量和ONJ的发生率。与对照组相比,DPSC-CM处理组小鼠拔牙窝周围组织中炎性细胞因子的表达减弱,而成骨相关分子的表达增强。此外,被抑制的 Wnt 信号分子的表达也得到了改善。这些发现共同表明,DPSC-CM 可预防小鼠 DRONJ 模型中 ONJ 的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Preventive Effects of Dental Pulp Stem Cell-conditioned Media on Anti-RANKL Antibody-Related Osteonecrosis of the Jaw.

Preventive Effects of Dental Pulp Stem Cell-conditioned Media on Anti-RANKL Antibody-Related Osteonecrosis of the Jaw.

Medication-related osteonecrosis of the jaw is a serious disease occurring in patients with cancer and osteoporosis, who are undergoing treatment with antiresorptive agents (ARAs) such as bisphosphonate (BP) or denosumab, an antibody targeting receptor activator of NF-κB ligand. Recently, stem cell-based therapy has been shown to be effective in preventing the development of bisphosphonate-related osteonecrosis of the jaw. However, studies on denosumab-related osteonecrosis of the jaw (DRONJ) remain limited. Here, the efficacy of treatment with dental pulp stem cell conditioned media (DPSC-CM) in preventing DRONJ in a murine model was evaluated. Local administration of DPSC-CM into the extraction socket of a mouse with DRONJ decreased the number of empty osteocyte lacunae and the prevalence of ONJ. In tissues surrounding the extraction sockets in the DPSC-CM-treated group, the expression of inflammatory cytokines was attenuated and that of osteogenesis-related molecules was enhanced compared to that in the control group. Further, the expression of Wnt signaling molecules, which had been suppressed, was improved. These findings collectively suggest that DPSC-CM prevents ONJ development in a murine DRONJ model.

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来源期刊
Calcified Tissue International
Calcified Tissue International 医学-内分泌学与代谢
CiteScore
8.00
自引率
2.40%
发文量
112
审稿时长
4-8 weeks
期刊介绍: Calcified Tissue International and Musculoskeletal Research publishes original research and reviews concerning the structure and function of bone, and other musculoskeletal tissues in living organisms and clinical studies of musculoskeletal disease. It includes studies of cell biology, molecular biology, intracellular signalling, and physiology, as well as research into the hormones, cytokines and other mediators that influence the musculoskeletal system. The journal also publishes clinical studies of relevance to bone disease, mineral metabolism, muscle function, and musculoskeletal interactions.
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