熊果和花萃取物对暴露于钴-60 源的四膜虫的潜在影响

Q4 Veterinary
Fatine Belfekih, Ahmed Moussaif, Mohammed El Mzibri, Adnane Moutaouakkil, Laila Benbacer, Rachid Bengueddour, Abdelghani Iddar
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引用次数: 0

摘要

将梨形四膜虫培养物暴露于钴-60 72 小时,会对细胞的生长、外观和生理产生显著影响。本研究旨在探讨熊果花和果实提取物对吡喃四甲虫抗伽马辐射的保护作用。首先,制备了果实和花的水提取物和 50%乙醇提取物,并评估了它们对纤毛虫的细胞毒性。在生长培养基中加入无毒浓度为 25 µg/mL 的萃取物后,辐照纤毛虫的细胞活力和形态都得到了改善。加入萃取物后,甘油醛-3-磷酸脱氢酶和琥珀酸脱氢酶的活性恢复到了初始水平,与未受辐照的细胞相似。此外,提取物还降低了氧化应激指标,如脂质过氧化,并降低了抗氧化防御酶、过氧化氢酶和超氧化物歧化酶的活性。这可能归因于萃取物的抗氧化特性。研究结果表明,花提取物比果提取物具有更好的保护作用,在体外 DPPH 清除试验中具有更强的抗氧化活性。这些结果表明,A. unedo 花提取物可能具有作为外源性辐射防护剂的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potential effect of fruit and flower extracts of Arbutus unedo L. on Tetrahymena pyriformis exposed to a cobalt-60 source
Exposure of Tetrahymena pyriformis cultures to cobalt-60 for 72 h significantly impacted the cells' growth, appearance, and physiology. This study aims to investigate the protective effects of Arbutus unedo L flowers and fruit extracts on T. pyriformis against gamma radiation. Initially, aqueous and 50% ethanolic extracts of the fruits and flowers were prepared, and their cytotoxicity on the ciliate was evaluated. The irradiated ciliate's cellular viability and morphological aspect improved when a non-toxic concentration of 25 µg/mL was added to the growth medium. The addition of extracts restored glyceraldehyde-3-phosphate dehydrogenase and succinate dehydrogenase activities to their initial levels, similar to non-irradiated cells. In addition, the extracts reduced oxidative stress markers, such as lipid peroxidation, and decreased the activities of antioxidant defence enzymes, catalase, and superoxide dismutase. This may be attributed to the antioxidant properties of the extracts. Results of this study revealed that the flower extracts exhibited better protective effects than the fruit extracts, with superior antioxidant activity in the in-vitro DPPH scavenging assay. These results suggest that A. unedo flower extracts may have potential as exogenous radioprotective agents.
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来源期刊
Journal of Experimental Biology and Agricultural Sciences
Journal of Experimental Biology and Agricultural Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.00
自引率
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发文量
127
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