通过患者自我采样与临床医生采样进行 HPV DNA 基因分型测试的一致性和可接受性

Q4 Medicine
J. Indarti, Danny Maesadatu Syaharutsa, Ilham Utama Surya, Kristian Alda
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引用次数: 0

摘要

目标:确定这种替代方法的有效性,尤其是在 COVID-19 大流行期间,并考虑到印度尼西亚的文化背景。方法本研究采用横断面设计,涉及 Cipto Mangunkusumo 医生综合医院妇科和阴道镜诊所的患者。样本量估计为 48 个,根据诊断测试公式确定。样本人群包括 VIA 阳性或巴氏涂片结果异常的女性患者。每位患者都使用 GenoFlow HPV 阵列技术进行了 HPV DNA 自我采样和临床医生采样检测,并继续接受阴道镜检查。所有患者还接受了一份由八个问题组成的问卷调查,内容涉及他们对HPV DNA自我采样检测的看法。数据分析采用 SPSS 20 版的 2 × 2 表,并计算了科恩卡帕系数,以衡量患者和临床医生采样结果的一致性。结果在临床医生进行的检查中,有 33 名患者的 HPV 结果呈阳性,而通过自我采样,有 28 名患者的 HPV 结果呈阳性(P=0.00)。最常观察到的是高危 HPV,其中以 HPV 16 型最多(15%)。根据这些数据,自我采样的敏感性、特异性、阳性预测值和阴性预测值分别为 85%、100%、100% 和 75%,吻合率为 89.6%。临床医生采样与自我采样之间的科恩卡帕系数(Cohen's Kappa coefficient)为 K=0.778,吻合度较高(K=0.61-0.80)。所有患者都认为操作简单(100%),大多数患者(60.5%)表示更喜欢自我取样法。结论: 自我采样与临床医生采样检测 HPV DNA 的结果有很好的一致性,患者积极接受自我采样方法,表明其有可能成为一种有效的宫颈癌筛查方法。关键词:宫颈癌筛查宫颈癌筛查 临床医生采样 人类乳头瘤病毒 自我采样
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Concordance and Acceptability of HPV DNA Genotyping Test by Patient’s Self-Sampling Against Clinician Sampling
Objectives: To determine the effectiveness of this alternative method, especially during the COVID-19 pandemic and considering Indonesia’s cultural context. Methods: This study utilized a cross-sectional design, and involved patients at the Gynecology and Colposcopy Clinic of Dr. Cipto Mangunkusumo General Hospital. The estimated sample size was 48, determined using a diagnostic test formula. The sample population consisted of female patients with positive VIA or abnormal Pap smear results. Each patient underwent HPV DNA self-sampling and clinician sampling tests using the GenoFlow HPV Array technique and continued with colposcopy. All patients were also administered a questionnaire consisting of eight questions about their perspective on the self-sampling HPV DNA test. The data analysis employed a 2 × 2 table using SPSS version 20, and Cohen’s kappa coefficient was calculated to measure the agreement between the sampling results of patients’ and Clinicians’. Results: Among the examinations conducted by clinicians, there were 33 patients with positive HPV results, whereas through self-sampling, there were 28 patients with positive HPV (p=0.00). High risk HPV was the most commonly observed, with HPV type 16 appearing the most (15%). Based on these data, the self-sampling sensitivity, specificity, positive predictive value, and negative predictive value were 85%, 100%, 100%, and 75%, respectively, with a concordance rate of 89.6%. The Cohen’s Kappa coefficient between samples taken by the clinician and self-sampling resulted in K=0.778, which is considered a good agreement (K=0.61-0.80). All patients concluded that the procedure was easy (100%), and the majority (60.5%) expressed a preference for the self-sampling method. Conclusion:  There is a good agreement between the results of self-sampling and clinician sampling for detecting HPV DNA, with patients positively accepting the self-sampling method, indicating its potential as an effective cervical cancer screening method. Keywords: Cervical Cancer Screening, Clinician Sampling, Human Papillomavirus, Self-Sampling.
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来源期刊
Indonesian Journal of Obstetrics and Gynecology
Indonesian Journal of Obstetrics and Gynecology Medicine-Pathology and Forensic Medicine
CiteScore
0.10
自引率
0.00%
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0
审稿时长
24 weeks
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