轻度至中度干旱胁迫强化了功能微生物群在促进荒漠草原优势牧草物种(Neopallasia pectinata)生长中的作用

Hui Gao, Zhenzhen Huang, Weiwei Chen, An Xing, Shixiang Zhao, Weifan Wan, Haina Hu, Haigang Li
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摘要

沙漠草原生态系统很容易受到干旱胁迫,从而影响草原的生态平衡和可持续发展。干旱胁迫除了直接限制植物生长外,还通过改变根相关微生物群的多样性和功能间接影响植物的适应性。我们进行了一项盆栽实验,以评估合成微生物群落(SynComs)在改善中国内蒙古荒漠草原上 Neopallasia pectinata (Pall.) Poljak 的植物生长和抗旱性方面的作用。从荒漠草原上 12 种主要牧草的根圈和根内圈中提取了 6 种 SynComs。每个合成菌群包括两到三个细菌属(芽孢杆菌属、原生单胞菌属和链霉菌属)。在无水胁迫(土壤持水量为 75%,WHC)条件下,单一菌株在促进植物生长(茎杆直径、根长和植物干重)方面的表现优于 SynComs,其中对 Streptomyces coeruleorubidus ATCC 13740 的影响最大(p < 0.05)。然而,在轻度至中度干旱胁迫下(55% 和 35% WHC),SynComs 在提高植物生物量积累和诱导产生抗性相关物质方面的表现优于单一菌株(p < 0.05)。在极端干旱胁迫(20% WHC)下,单一菌株和 SynComs 均无明显效果。这项研究强调了 SynComs 在促进荒漠草原牧草植物抗旱方面的潜力。轻度至中度干旱胁迫刺激 SynComs 有利于 N. pectinata 植物的生长,尽管微生物效应存在土壤水分阈值(21% WHC)。通过操纵饲料植物的功能微生物组,SynComs 的使用为荒漠草原的生态恢复和可持续利用提供了一种前景广阔的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mild to moderate drought stress reinforces the role of functional microbiome in promoting growth of a dominant forage species (Neopallasia pectinata) in desert steppe
Desert steppe ecosystems are prone to drought stress, which influences the ecological balance and sustainable development of grasslands. In addition to directly restrict plant growth, drought stress indirectly impacts plant fitness by altering the diversity and function of root-associated microbiomes. This begs the question of whether the functional microbiome of forage plants, represented by synthetic microbial communities (SynComs), can be leveraged to mitigate drought stress in desert steppes and promote the ecological restoration of these fragile ecosystems.A pot experiment was conducted to evaluate the role of SynComs in improving the plant growth and drought stress resistance of Neopallasia pectinata (Pall.) Poljak in desert steppe in Inner Mongolia, China. Six SynComs were derived from the rhizosphere and root endosphere of 12 dominant forage species in the desert steppe. Each SynCom comprised two to three bacterial genera (Bacillus, Protomicromonospora, and Streptomyces). We examined the capacities of different SynComs for nutrient solubilization, phytohormone secretion, and enzymatic activity.Under no water stress (75% soil water holding capacity, WHC), single strains performed better than SynComs in promoting plant growth in terms of stem diameter, root length, and plant dry weight, with the greatest effects observed for Streptomyces coeruleorubidus ATCC 13740 (p < 0.05). However, under mild to moderate drought stress (55% and 35% WHC), SynComs outperformed single strains in enhancing plant biomass accumulation and inducing the production of resistance-related substances (p < 0.05). No significant effect of single strains and SynComs emerged under extreme drought stress (20% WHC).This study underscores the potential of SynComs in facilitating forage plants to combat drought stress in desert steppe. Mild to moderate drought stress stimulates SynComs to benefit the growth of N. pectinata plants, despite a soil moisture threshold (21% WHC) exists for the microbial effect. The use of SynComs provides a promising strategy for the ecological restoration and sustainable utilization of desert steppes by manipulating the functional microbiome of forage plants.
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