亚临床牛乳腺炎中金黄色葡萄球菌微生物诊断对无乳链球菌闪电疗法的干扰

Ana Flávia Novaes Gomes, Fúlvia de Fátima Almeida de Castro, Márcio Roberto Silva, Carla Christine Lange, J. B. Ribeiro, Alessandro de Sá Guimarães, Guilherme Nunes de Souza
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摘要

牛乳腺炎是奶牛的一种重要疾病,代价高昂。巴西通常采用的诊断方法是体细胞计数(SCC)和牛奶微生物学检测。牛奶中的细菌脱落率低,意味着微生物检测中没有菌落生长,结果为假阴性。无乳链球菌和金黄色葡萄球菌是混合型乳腺炎的主要病原体。然而,与金黄色葡萄球菌相比,无乳链球菌从乳腺中释放的细菌更多,从而影响了诊断金黄色葡萄球菌的微生物敏感性。本研究旨在根据牛奶中分离出的病原体估算奶牛的SCC和细菌总数(TBC),并评估在治疗无乳乳酸杆菌(即闪击疗法)期间通过微生物检测诊断金黄色葡萄球菌的差异。虽然 S. agalactiae 和 S. aureus 都显示出较高的 SCC 平均值,但 S. agalactiae 的细菌脱落率是 S. aureus 的 2.3 倍。在对无乳酸杆菌进行治疗的 5 个月中,金黄色葡萄球菌的微生物敏感性有所提高。治疗 5 个月后,金黄色葡萄球菌的感染率有所下降。金黄色葡萄球菌的感染率上升到 39.0。结果表明,由于聚合酶链式反应(PCR)灵敏度高,可在突击疗法开始时使用,目的是在奶牛群中根除半乳链球菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interference of Streptococcus agalactiae Blitz Therapy in Staphylococcus aureus Microbiological Diagnosis in Subclinical Bovine Mastitis
Bovine mastitis is an important and costly disease to dairy cattle. Diagnostic methods usually performed in Brazil are somatic cell counts (SCC) and milk microbiology. Low bacteria shedding in milk implies no colony growth in microbiological tests and false negative results. Streptococcus agalactiae and Staphylococcus aureus are principal pathogens of mixed mastitis. However, S. agalactiae has a higher bacterial release from the mammary gland than S. aureus, affecting microbiological sensitivity to diagnose S. aureus. This study aimed to estimate the SCC and total bacterial count (TBC) from cows according to pathogen isolated in milk and to evaluate variation in S. aureus diagnosis by a microbiological test during S. agalactiae treatment, which is called blitz therapy. Both S. agalactiae and S. aureus presented high SCC means, although S. agalactiae showed shedding of bacteria 2.3 times greater than S. aureus. Microbiological sensitivity to S. aureus increased for 5 months during S. agalactiae treatment. The prevalence of S. agalactiae fell after 5 months of therapeutic procedures. The prevalence of S. aureus increased to 39.0. The results showed that due to high sensitivity, the polymerase chain reaction (PCR) could be used at the beginning of blitz therapy with the goal of S. agalactiae eradication from the dairy herd.
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