从泰国驯养的家养蟋蟀 Acheta domesticus（直翅目：蝼蛄科）中分离出的新伏病毒

IF 4.7 3区农林科学 Q1 ENTOMOLOGY

Journal of Insects as Food and Feed Pub Date : 2024-05-23 DOI:10.1163/23524588-00001217

S. Homchan, Y.M. Gupta

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引用次数: 0

摘要

本研究调查了商业化蟋蟀养殖过程中Acheta domesticus volvovirus（AdVV）的基因组特征。对Acheta domesticus基因组的BLAST分析发现了与AdVVV-Japan基因组高度相似的序列，表明AdVVV的存在。聚合酶链式反应（PCR）证实了泰国那空叻丕岛农场的A. domesticus种群感染了AdVV病毒。通过定向引物扩增和测序，重建了完整的 2,516 个核苷酸的 AdVV-Thailand 基因组。基因组包含四个开放阅读框，编码假定蛋白，末端具有特征性发夹结构，与其他 AdVV 分离物一致。系统发育分析表明，与其他分离物相比，AdVVV-泰国与 AdVVV-日本的遗传关系更为密切。对 5 个 AdVV 病毒分离株的编码序列进行比较分析后发现，假定蛋白/顶盖蛋白 ORF1 的变异性最高，1086 个碱基中有 64 个变异位点，这表明它在遗传多样性方面具有重要意义。相比之下，ORF2、ORF3 和 ORF4 的变异性最小。大多数变异都是单倍变异，其中 85.33% 局限于 ORF1。这项研究证实了商业蟋蟀养殖场中存在 AdVVV，重建了 AdVV-Thailand 基因组，深入了解了其系统发育和不同分离株之间的遗传多样性，强调了假定的噬菌体蛋白在驱动变异中的作用。这些发现加深了人们对 AdVVV 基因组学和蟋蟀种群内进化动态的了解。

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New volvovirus isolate from Thai domesticated house cricket, Acheta domesticus (Orthoptera: Gryllidae)

This study investigated the genomic characteristics of Acheta domesticus volvovirus (AdVVV) in a commercial cricket farming operation. BLAST analysis of the Acheta domesticus genome assembly identified sequences with high similarity to the AdVVV-Japan genome, suggesting AdVVV presence. PCR confirmed AdVVV infection in the A. domesticus breeding population from Nakhon Ratchasima farm, Thailand. The complete 2,516 nucleotide AdVVV-Thailand genome was reconstructed through targeted primer amplification and sequencing. It contained four open reading frames encoding hypothetical proteins, with a characteristic hairpin structure at the termini, consistent with other AdVVV isolates. Phylogenetic analysis revealed AdVVV-Thailand’s closer genetic affiliation with AdVVV-Japan compared to other isolates. Comparative analysis of coding sequences across five AdVVV isolates showed the highest variability in the hypothetical protein/putative capsid protein ORF1, with 64 variable sites out of 1086 bases, suggesting its significance in genetic diversity. In contrast, ORF2, ORF3, and ORF4 exhibited minimal variability. The majority of variations were singletons, with 85.33% confined to ORF1. This study confirmed AdVVV presence in a commercial cricket farm, reconstructed the AdVVV-Thailand genome, provided insights into its phylogeny and genetic diversity across isolates, highlighting the putative capsid protein’s role in driving variability. These findings enhance understanding of AdVVV genomics and evolutionary dynamics within cricket populations.

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来源期刊

Journal of Insects as Food and Feed Agricultural and Biological Sciences-Insect Science

CiteScore

7.00

自引率

17.60%

发文量

133

期刊介绍： The Journal of Insects as Food and Feed covers edible insects from harvesting in the wild through to industrial scale production. It publishes contributions to understanding the ecology and biology of edible insects and the factors that determine their abundance, the importance of food insects in people’s livelihoods, the value of ethno-entomological knowledge, and the role of technology transfer to assist people to utilise traditional knowledge to improve the value of insect foods in their lives. The journal aims to cover the whole chain of insect collecting or rearing to marketing edible insect products, including the development of sustainable technology, such as automation processes at affordable costs, detection, identification and mitigating of microbial contaminants, development of protocols for quality control, processing methodologies and how they affect digestibility and nutritional composition of insects, and the potential of insects to transform low value organic wastes into high protein products. At the end of the edible insect food or feed chain, marketing issues, consumer acceptance, regulation and legislation pose new research challenges. Food safety and legislation are intimately related. Consumer attitude is strongly dependent on the perceived safety. Microbial safety, toxicity due to chemical contaminants, and allergies are important issues in safety of insects as food and feed. Innovative contributions that address the multitude of aspects relevant for the utilisation of insects in increasing food and feed quality, safety and security are welcomed.