对不同收获期桑黄菌菌丝体和子实体的广泛靶向代谢组学分析

Yue Qi, Xiao-Ying Guo, Xin-Yue Xu, Jian-Xuan Hou, Shi-Lai Liu, Hong-Bo Guo, Ai-Guo Xu, Rui-Heng Yang, Xiao-Dan Yu
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引用次数: 0

摘要

万年青(Sanghuangprous vaninii)是一种在中国广泛栽培的药用大型真菌。香荚兰的菌丝体和子实体都具有显著的治疗功效,但菌丝体是否可以替代子实体仍不清楚。此外,万年青是一种多年生真菌,其治疗成分因子实体生长年份的不同而有很大差异。因此,为特定目的选择合适的香荚兰子实体采收期至关重要。借助中药系统药理学数据库和分析平台(TCMSP),采用基于超高效液相色谱-三重四极杆质谱(UHPLC-QQQ-MS)的代谢组学方法,初步确定了香附子中对六种主要疾病具有抗性的81种关键活性代谢物和157种活性药物代谢物。为了评估万年青菌丝体和子实体的可替代性,并选择万年青子实体的适当采收期,我们分析了三个不同采收期(1 年、2 年和 3 年)的菌丝体和子实体之间的代谢物差异,尤其是活性代谢物差异。此外,我们还确定了每个万年青样本中最主要和最关键的代谢物。这些结果表明,菌丝体有望成为香豌豆子实体的替代品,而且延长生长年限并不一定会导致香豌豆子实体中活性代谢物的积累水平提高。这项研究为开发和利用万年青子实体提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Widely targeted metabolomics analysis of Sanghuangporus vaninii mycelia and fruiting bodies at different harvest stages
Sanghuangprous vaninii is a medicinal macrofungus cultivated extensively in China. Both the mycelia and fruiting bodies of S. vaninii have remarkable therapeutic properties, but it remains unclear whether the mycelia may serve as a substitute for the fruiting bodies. Furthermore, S. vaninii is a perennial fungus with therapeutic components that vary significantly depending on the growing year of the fruiting bodies. Hence, it is critical to select an appropriate harvest stage for S. vaninii fruiting bodies for a specific purpose. With the aid of Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), metabolomics based on ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry (UHPLC-QQQ-MS) was used to preliminarily determine 81 key active metabolites and 157 active pharmaceutical metabolites in S. vaninii responsible for resistance to the six major diseases. To evaluate the substitutability of the mycelia and fruiting bodies of S. vaninii and to select an appropriate harvest stage for the fruiting bodies of S. vaninii, we analyzed the metabolite differences, especially active metabolite differences, among the mycelia and fruiting bodies during three different harvest stages (1-year-old, 2-year-old, and 3-year-old). Moreover, we also determined the most prominent and crucial metabolites in each sample of S. vaninii. These results suggested that the mycelia show promise as a substitute for the fruiting bodies of S. vaninii and that extending the growth year does not necessarily lead to higher accumulation levels of active metabolites in the S. vaninii fruiting bodies. This study provided a theoretical basis for developing and using S. vaninii.
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