土耳其健康绵羊中巴贝斯虫的血清学和分子学调查

Mehmet Bozan, Mehmet Can Ulucesme, Arda Eyvaz, O. Ceylan, F. Sevinc, M. Aktas, S. Ozubek
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引用次数: 0

摘要

由巴贝斯虫(Babesia ovis)引起的巴贝斯虫病是绵羊的一个主要季节性问题,尤其是在土耳其等蜱虫数量较多的国家。以往采用显微镜、血清学或分子技术等不同方法进行的研究报告了有关绵羊巴贝西亚原虫病的不同流行病学数据。为了填补这一知识空白,我们的研究采用了巢式 PCR(nPCR)/间接 ELISA(iELISA)相结合的方法,使用这两种技术分析了 2023 年 4 月至 7 月间从 414 只绵羊身上采集的血液样本。相反,使用 iELISA 针对 BoSA1 抗原进行血清学检测,结果显示抗巴贝斯虫抗体阳性率明显更高,达到 59.9%。恢复后巴贝斯虫的暂时存在降低了 nPCR 的灵敏度,导致分子流行率降低。然而,即使宿主体内不存在巴贝西亚原虫,抗巴贝西亚原虫抗体也会在血清中保留很长时间,并可通过血清学方法检测到。我们的研究强调了同时采用分子和血清学方法准确评估巴贝虫流行率的必要性。它强调了综合流行病学方法对有效管理羊群疾病的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serological and Molecular Survey of Babesia ovis in Healthy Sheep in Türkiye
Babesiosis, caused by Babesia ovis, is a major seasonal issue in sheep, particularly in countries like Türkiye with high Rhipicephalus bursa tick populations. Previous studies employing various methods such as microscopy, serology, or molecular techniques have reported different epidemiological data concerning ovine babesiosis. Addressing this knowledge gap, our study employed a combined nested PCR (nPCR)/indirect ELISA (iELISA) approach, analyzing blood samples collected from 414 sheep between April and July 2023 using both techniques. nPCR amplified the 18S ribosomal RNA gene of B. ovis and determined a molecular prevalence of 1.9%. Conversely, serological testing using iELISA targeted the BoSA1 antigen and revealed a significantly higher positivity rate of 59.9% for anti-B. ovis antibodies. The temporary presence of Babesia after recovery reduces nPCR sensitivity, resulting in lower molecular prevalence. However, even if Babesia is not present in the host, anti-B. ovis antibodies remain in the serum for a long time and can be detected serologically. Our study underscores the necessity of concurrently employing molecular and serological methods for an accurate assessment of B. ovis prevalence. It highlights the importance of comprehensive epidemiological approaches for effective disease management in sheep populations.
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