Daniel J Leybourne, Mark A Whitehead, Torsten Will
{"title":"病媒种群的遗传多样性影响一种重要植物病毒的传播效率。","authors":"Daniel J Leybourne, Mark A Whitehead, Torsten Will","doi":"10.1098/rsbl.2024.0095","DOIUrl":null,"url":null,"abstract":"<p><p>The transmission efficiency of aphid-vectored plant viruses can differ between aphid populations. Intra-species diversity (genetic variation, endosymbionts) is a key determinant of aphid phenotype; however, the extent to which intra-species diversity contributes towards variation in virus transmission efficiency is unclear. Here, we use multiple populations of two key aphid species that vector barley yellow dwarf virus (BYDV) strain PAV (BYDV-PAV), the grain aphid (<i>Sitobion avenae</i>) and the bird cherry-oat aphid (<i>Rhopalosiphum padi</i>), and examine how diversity in vector populations influences virus transmission efficiency. We use Illumina sequencing to characterize genetic and endosymbiont variation in multiple <i>Si. avenae</i> and <i>Rh. padi</i> populations and conduct BYDV-PAV transmission experiments to identify links between intra-species diversity in the vector and virus transmission efficiency. We observe limited variation in the transmission efficiency of <i>Si. avenae,</i> with transmission efficiency consistently low for this species. However, for <i>Rh. padi,</i> we observe a range of transmission efficiencies and show that BYDV transmission efficiency is influenced by genetic diversity within the vector, identifying 542 single nucleotide polymorphisms that potentially contribute towards variable transmission efficiency in <i>Rh. padi</i>. Our results represent an important advancement in our understanding of the relationship between genetic diversity, vector-virus interactions, and virus transmission efficiency.</p>","PeriodicalId":9005,"journal":{"name":"Biology Letters","volume":"20 5","pages":"20240095"},"PeriodicalIF":2.8000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11285819/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genetic diversity in vector populations influences the transmission efficiency of an important plant virus.\",\"authors\":\"Daniel J Leybourne, Mark A Whitehead, Torsten Will\",\"doi\":\"10.1098/rsbl.2024.0095\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The transmission efficiency of aphid-vectored plant viruses can differ between aphid populations. Intra-species diversity (genetic variation, endosymbionts) is a key determinant of aphid phenotype; however, the extent to which intra-species diversity contributes towards variation in virus transmission efficiency is unclear. Here, we use multiple populations of two key aphid species that vector barley yellow dwarf virus (BYDV) strain PAV (BYDV-PAV), the grain aphid (<i>Sitobion avenae</i>) and the bird cherry-oat aphid (<i>Rhopalosiphum padi</i>), and examine how diversity in vector populations influences virus transmission efficiency. We use Illumina sequencing to characterize genetic and endosymbiont variation in multiple <i>Si. avenae</i> and <i>Rh. padi</i> populations and conduct BYDV-PAV transmission experiments to identify links between intra-species diversity in the vector and virus transmission efficiency. We observe limited variation in the transmission efficiency of <i>Si. avenae,</i> with transmission efficiency consistently low for this species. However, for <i>Rh. padi,</i> we observe a range of transmission efficiencies and show that BYDV transmission efficiency is influenced by genetic diversity within the vector, identifying 542 single nucleotide polymorphisms that potentially contribute towards variable transmission efficiency in <i>Rh. padi</i>. Our results represent an important advancement in our understanding of the relationship between genetic diversity, vector-virus interactions, and virus transmission efficiency.</p>\",\"PeriodicalId\":9005,\"journal\":{\"name\":\"Biology Letters\",\"volume\":\"20 5\",\"pages\":\"20240095\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11285819/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biology Letters\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1098/rsbl.2024.0095\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/22 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology Letters","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1098/rsbl.2024.0095","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/22 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
Genetic diversity in vector populations influences the transmission efficiency of an important plant virus.
The transmission efficiency of aphid-vectored plant viruses can differ between aphid populations. Intra-species diversity (genetic variation, endosymbionts) is a key determinant of aphid phenotype; however, the extent to which intra-species diversity contributes towards variation in virus transmission efficiency is unclear. Here, we use multiple populations of two key aphid species that vector barley yellow dwarf virus (BYDV) strain PAV (BYDV-PAV), the grain aphid (Sitobion avenae) and the bird cherry-oat aphid (Rhopalosiphum padi), and examine how diversity in vector populations influences virus transmission efficiency. We use Illumina sequencing to characterize genetic and endosymbiont variation in multiple Si. avenae and Rh. padi populations and conduct BYDV-PAV transmission experiments to identify links between intra-species diversity in the vector and virus transmission efficiency. We observe limited variation in the transmission efficiency of Si. avenae, with transmission efficiency consistently low for this species. However, for Rh. padi, we observe a range of transmission efficiencies and show that BYDV transmission efficiency is influenced by genetic diversity within the vector, identifying 542 single nucleotide polymorphisms that potentially contribute towards variable transmission efficiency in Rh. padi. Our results represent an important advancement in our understanding of the relationship between genetic diversity, vector-virus interactions, and virus transmission efficiency.
期刊介绍:
Previously a supplement to Proceedings B, and launched as an independent journal in 2005, Biology Letters is a primarily online, peer-reviewed journal that publishes short, high-quality articles, reviews and opinion pieces from across the biological sciences. The scope of Biology Letters is vast - publishing high-quality research in any area of the biological sciences. However, we have particular strengths in the biology, evolution and ecology of whole organisms. We also publish in other areas of biology, such as molecular ecology and evolution, environmental science, and phylogenetics.