Ze Yang, Lili Xie, Jingjing Ba, Simin Zan, Letong Zhang, Xinyi Zhang, Yang Yu
{"title":"通过血栓弹力图对人类和金色叙利亚仓鼠进行比较研究。","authors":"Ze Yang, Lili Xie, Jingjing Ba, Simin Zan, Letong Zhang, Xinyi Zhang, Yang Yu","doi":"10.1002/ame2.12403","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Thromboelastography (TEG) is a widely utilized clinical testing method for real-time monitoring of platelet function and the thrombosis process. Lipid metabolism disorders are crucial risk factors for thrombosis. The lipid metabolism characteristics of hamsters resemble those of humans more closely than mice and rats, and their relatively large blood volume makes them suitable for studying the mechanisms of thrombosis related to plasma lipid mechanisms. Whole blood samples from golden Syrian hamsters and healthy humans were obtained following standard clinical procedures. TEG was employed to evaluate coagulation factor function, fibrinogen (Fib) function, platelet function, and the fibrinolytic system.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>The whole blood from hamster or healthy human was isolated following the clinical procedure, and TEG was employed to evaluate the coagulation factor function, Fib function, platelet function, and fibrinolytic system. Coagulation analysis used ACLTOP750 automatic coagulation analysis pipeline. Blood routine testing used XN-2000 automatic blood analyzer.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>TEG parameters revealed that hamsters exhibited stronger coagulation factor function than humans (reaction time [R], <i>p</i> = 0.0117), with stronger Fib function (alpha angle, <i>p</i> < 0.0001; K-time [K], <i>p</i> < 0.0001). Platelet function did not differ significantly (maximum amplitude [MA], <i>p</i> = 0.077). Hamsters displayed higher coagulation status than humans (coagulation index [CI], <i>p</i> = 0.0023), and the rate of blood clot dissolution in hamsters differed from that in humans (percentage lysis 30 min after MA, <i>p</i> = 0.02). Coagulation analysis parameters indicated that prothrombin time (PT) and activated partial thromboplastin time (APTT) were faster in hamsters than in humans (PT, <i>p</i> = 0.0014; APTT, <i>p</i> = 0.03), whereas the Fib content was significantly lower in hamsters than in humans (<i>p</i> < 0.0001). No significant difference was observed in thrombin time (<i>p</i> = 0.1949).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>In summary, TEG could be used to evaluate thrombosis and bleeding parameters in whole blood samples from hamsters. The platelet function of hamsters closely resembled that of humans, whereas their coagulation function was significantly stronger.</p>\n </section>\n </div>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"7 4","pages":"570-577"},"PeriodicalIF":0.0000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12403","citationCount":"0","resultStr":"{\"title\":\"Comparative studies between humans and golden Syrian hamsters via thromboelastography\",\"authors\":\"Ze Yang, Lili Xie, Jingjing Ba, Simin Zan, Letong Zhang, Xinyi Zhang, Yang Yu\",\"doi\":\"10.1002/ame2.12403\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Thromboelastography (TEG) is a widely utilized clinical testing method for real-time monitoring of platelet function and the thrombosis process. Lipid metabolism disorders are crucial risk factors for thrombosis. The lipid metabolism characteristics of hamsters resemble those of humans more closely than mice and rats, and their relatively large blood volume makes them suitable for studying the mechanisms of thrombosis related to plasma lipid mechanisms. Whole blood samples from golden Syrian hamsters and healthy humans were obtained following standard clinical procedures. TEG was employed to evaluate coagulation factor function, fibrinogen (Fib) function, platelet function, and the fibrinolytic system.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>The whole blood from hamster or healthy human was isolated following the clinical procedure, and TEG was employed to evaluate the coagulation factor function, Fib function, platelet function, and fibrinolytic system. Coagulation analysis used ACLTOP750 automatic coagulation analysis pipeline. Blood routine testing used XN-2000 automatic blood analyzer.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>TEG parameters revealed that hamsters exhibited stronger coagulation factor function than humans (reaction time [R], <i>p</i> = 0.0117), with stronger Fib function (alpha angle, <i>p</i> < 0.0001; K-time [K], <i>p</i> < 0.0001). Platelet function did not differ significantly (maximum amplitude [MA], <i>p</i> = 0.077). Hamsters displayed higher coagulation status than humans (coagulation index [CI], <i>p</i> = 0.0023), and the rate of blood clot dissolution in hamsters differed from that in humans (percentage lysis 30 min after MA, <i>p</i> = 0.02). Coagulation analysis parameters indicated that prothrombin time (PT) and activated partial thromboplastin time (APTT) were faster in hamsters than in humans (PT, <i>p</i> = 0.0014; APTT, <i>p</i> = 0.03), whereas the Fib content was significantly lower in hamsters than in humans (<i>p</i> < 0.0001). No significant difference was observed in thrombin time (<i>p</i> = 0.1949).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>In summary, TEG could be used to evaluate thrombosis and bleeding parameters in whole blood samples from hamsters. The platelet function of hamsters closely resembled that of humans, whereas their coagulation function was significantly stronger.</p>\\n </section>\\n </div>\",\"PeriodicalId\":93869,\"journal\":{\"name\":\"Animal models and experimental medicine\",\"volume\":\"7 4\",\"pages\":\"570-577\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12403\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal models and experimental medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/ame2.12403\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Health Professions\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal models and experimental medicine","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ame2.12403","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Health Professions","Score":null,"Total":0}
Comparative studies between humans and golden Syrian hamsters via thromboelastography
Background
Thromboelastography (TEG) is a widely utilized clinical testing method for real-time monitoring of platelet function and the thrombosis process. Lipid metabolism disorders are crucial risk factors for thrombosis. The lipid metabolism characteristics of hamsters resemble those of humans more closely than mice and rats, and their relatively large blood volume makes them suitable for studying the mechanisms of thrombosis related to plasma lipid mechanisms. Whole blood samples from golden Syrian hamsters and healthy humans were obtained following standard clinical procedures. TEG was employed to evaluate coagulation factor function, fibrinogen (Fib) function, platelet function, and the fibrinolytic system.
Methods
The whole blood from hamster or healthy human was isolated following the clinical procedure, and TEG was employed to evaluate the coagulation factor function, Fib function, platelet function, and fibrinolytic system. Coagulation analysis used ACLTOP750 automatic coagulation analysis pipeline. Blood routine testing used XN-2000 automatic blood analyzer.
Results
TEG parameters revealed that hamsters exhibited stronger coagulation factor function than humans (reaction time [R], p = 0.0117), with stronger Fib function (alpha angle, p < 0.0001; K-time [K], p < 0.0001). Platelet function did not differ significantly (maximum amplitude [MA], p = 0.077). Hamsters displayed higher coagulation status than humans (coagulation index [CI], p = 0.0023), and the rate of blood clot dissolution in hamsters differed from that in humans (percentage lysis 30 min after MA, p = 0.02). Coagulation analysis parameters indicated that prothrombin time (PT) and activated partial thromboplastin time (APTT) were faster in hamsters than in humans (PT, p = 0.0014; APTT, p = 0.03), whereas the Fib content was significantly lower in hamsters than in humans (p < 0.0001). No significant difference was observed in thrombin time (p = 0.1949).
Conclusions
In summary, TEG could be used to evaluate thrombosis and bleeding parameters in whole blood samples from hamsters. The platelet function of hamsters closely resembled that of humans, whereas their coagulation function was significantly stronger.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
